Hydrogel Coating with Temperature Response Retention Behavior and Its Application in Selective Separation of Liquid
Fangbin Fan1,2, Xiaofeng Lu1, Licheng Wang1
1Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, China.
Abstract:
We reporte the double-layer hydrogel-coated mesoporous silica material as a new stationary phase for liquid chromatography. The method of combining physical coating and chemical coating was to apply hydrogel coating on the surface of silica, and finally, a new type of liquid chromatography stationary phase with in situ coating of the functional hydrogel on silica was obtained. This hydrogel-functionalized liquid chromatography stationary phase also exhibits a certain temperature responsiveness. Experimental results show that this temperature response is mainly due to changes in the hydrogen bonding between the stationary phase and the analyte at different temperatures in the column oven, which leads to changes in retention behavior. The hydrogel-coated mesoporous silica microspheres showed excellent selectivity for many polar analytes. An excellent column efficiency was obtained (139 000 plates/m for terephthalic acid) after optimization of chromatographic conditions. In addition to rapid separation of some analytes, this new hydrogel stationary phase also has certain superiority in chromatographic performance compared with other new excellent liquid chromatography stationary phases functioned by three-dimensional cross-linking systems. The important thing is that this strategy is relatively easy to prepare a new stationary phase with different properties.
More Related Videos
10:21Automated Hydrophobic Interaction Chromatography Column Selection for Use in Protein Purification
Published on: September 21, 2011
09:09Synthesis of PolyN-isopropylacrylamide Janus Microhydrogels for Anisotropic Thermo-responsiveness and Organophilic/Hydrophilic Loading Capability
Published on: February 27, 2016
Related Concept Videos
Silica Gel Column Chromatography: Overview
Polar components tend to bind strongly to the silica gel, causing them to move slowly through the column. In contrast, nonpolar compounds...
High-Performance Liquid Chromatography: Introduction
In HPLC, two phases play a critical role in the separation process:
High-Performance Liquid Chromatography: Instrumentation
High-Performance Liquid Chromatography: Elution Process
High-Performance Liquid Chromatography: Types of Detectors
Gas Chromatography: Types of Columns and Stationary Phases
For an analyte to remain on the column for a sufficient amount of time, it must exhibit some level of compatibility (or...
