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Zebrafish Model to Study Angiotensin II-Mediated Pathophysiology.
Bhagyashri Joshi1,2, Ganesh Wagh1,2, Harmandeep Kaur3
1Developmental Biology, Agharkar Research Institute, Pune 411004, India.
Biology
|November 27, 2021
Summary
Zebrafish models can now study hypertension. Angiotensin II injections in zebrafish induce cardiac remodeling, hypertrophy, and fibrosis, mirroring mammalian responses to aid hypertension research.
Area of Science:
- Cardiovascular Research
- Animal Models
- Hypertension Pathophysiology
Background:
- Hypertension damages vital organs like the kidney, heart, and brain.
- Understanding molecular pathways is key to managing hypertension.
- Animal models are crucial for studying disease mechanisms.
Purpose of the Study:
- To develop a zebrafish model for studying Angiotensin II-induced hypertension.
- To investigate the cardiac effects of Angiotensin II in zebrafish.
- To establish zebrafish as a model for Renin-Angiotensin System (RAS) signaling studies.
Main Methods:
- Adult zebrafish received intradermal Angiotensin II injections every 12 hours.
- Cardiac remodeling, cardiomyocyte hypertrophy, and cell proliferation were assessed.
- Gene expression of extracellular matrix (ECM) proteins and cardiac fibrosis were analyzed.
Main Results:
- Angiotensin II injection induced significant cardiac remodeling within seven days.
- Zebrafish cardiomyocytes exhibited hypertrophy and increased proliferation post-injection.
- Angiotensin II elevated ECM protein gene expression and induced fibrosis in cardiac ventricles.
Conclusions:
- Zebrafish respond to Angiotensin II similarly to mammals, showing cardiac pathogenesis.
- This zebrafish model effectively replicates Angiotensin II-induced cardiovascular changes.
- Zebrafish offer a viable model for studying AngII-RAS signaling and hypertension-related cardiovascular diseases.
