Related Experiment Video
Updated: Oct 12, 2025
09:20
An Adipocyte Cell Culture Model to Study the Impact of Protein and Micro-RNA Modulation on Adipocyte Function
Published on: May 4, 2021
3.9K
miR-370-3p Regulates Adipogenesis through Targeting Mknk1
Peiwen Zhang1,2, Xinrong Li1,2, Shunhua Zhang1,2
1College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, China.
Molecules (Basel, Switzerland)
|November 27, 2021
Summary
MicroRNA miR-370-3p inhibits fat accumulation by regulating adipogenesis and fatty acid metabolism. Lower miR-370-3p levels promote fat buildup, while its overexpression reduces it.
Area of Science:
- Biochemistry
- Molecular Biology
- Genetics
Background:
- Excessive fat accumulation is linked to obesity and metabolic diseases.
- MicroRNAs (miRNAs) are key regulators of gene expression, including those involved in fat metabolism.
- miRNAs can act as inhibitory factors against fat accumulation.
Purpose of the Study:
- To investigate the role of miR-370-3p in adipogenesis and fatty acid metabolism.
- To determine the effect of miR-370-3p expression levels on lipid accumulation.
- To identify the molecular targets and pathways regulated by miR-370-3p.
Main Methods:
- Comparative analysis of miR-370-3p expression in mice on high-fat vs. normal diets.
- Overexpression and inhibition of miR-370-3p in cell cultures (3T3-L1 preadipocytes) and in vivo.
- Measurement of adipogenic marker gene expression and lipid accumulation.
- Identification and validation of Mknk1 as a target gene of miR-370-3p.
Main Results:
- miR-370-3p expression was lower in mice fed a high-fat diet.
- Overexpression of miR-370-3p suppressed adipogenic markers and reduced lipid accumulation.
- Inhibition of miR-370-3p promoted preadipocyte proliferation and differentiation.
- Mknk1 was identified as a target gene, playing an opposing role in adipogenesis.
- miR-370-3p regulates fatty acid metabolism, potentially by modulating saturated and polyunsaturated fatty acid levels.
Conclusions:
- miR-370-3p plays a critical role in regulating adipogenesis.
- The miR-370-3p/Mknk1 axis is a key pathway in fatty acid metabolism.
- miR-370-3p represents a potential therapeutic target for obesity and related metabolic disorders.
More Related Videos
08:34Mechanism of Regulation of Adipocyte Numbers in Adult Organisms Through Differentiation and Apoptosis Homeostasis
Published on: June 3, 2016
15.3K
06:08Author Spotlight: Semi-Automated Isolation of the Stromal Vascular Fraction from Murine White Adipose Tissue Using a Tissue Dissociator
Published on: May 19, 2023
2.4K
Related Concept Videos
01:22PI3K/mTOR/AKT Signaling Pathway
4.1K
The mammalian target of rapamycin (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1 (mTORC1) and mTOR complex 2 (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast, mTORC2 consists of a...
4.1K
01:07MAPK Signaling Cascades
6.4K
Mitogen-activated protein kinase, or MAPK pathway, activates three sequential kinases to regulate cellular responses such as proliferation, differentiation, survival, and apoptosis. The canonical MAPK pathway starts with a mitogen or growth factor binding to an RTK. The activated RTKs stimulate Ras, which recruits Raf or MAP3 Kinase (MAPKKK), the first kinase of the MAPK signaling cascade. Raf further phosphorylates and activates MEK or MAP2 Kinases (MAPKK), which in turn phosphorylates MAP...
6.4K
03:03mTOR Signaling and Cancer Progression
3.9K
The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
The mTOR pathway or the...
3.9K
02:23Master Transcription Regulators
7.2K
Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
7.2K
01:58Cell Specific Gene Expression
14.2K
Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
14.2K
01:20The JAK-STAT Signaling Pathway
9.5K
Several cytokine receptors have tightly bound Janus kinase or JAK proteins attached at their cytosolic tail. Small signaling molecules such as cytokines, growth hormones, or prolactins bind to the cytokine receptors and initiate their dimerization. The dimerization brings the cytosolic JAKs together that trans-phosphorylate and activates each other. The activated JAKs now phosphorylate cytosolic tails of the cytokine receptors, which serve as binding sites for adaptor proteins such as SH2...
9.5K