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Charge Sensitive Optical Detection for Measurement of Small-Molecule Binding Kinetics.

Shaopeng Wang^1,2, Guangzhong Ma³, Runli Liang^3,4

¹Biodesign Center for Bioelectronics and Biosensors, Arizona State University, Tempe, AZ, USA. Shaopeng.Wang@asu.edu.

Methods in Molecular Biology (Clifton, N.J.)

|November 27, 2021

Summary

This summary is machine-generated.

Charge sensitive optical detection (CSOD) is a novel label-free method for real-time molecular interaction analysis. This technique accurately detects small molecules by measuring charge, not mass, enabling high-throughput drug screening.

Keywords:

Binding kinetics CSOD Charge sensitive optical detection Label free detection Optical fiber Optical imaging Small molecule

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Area of Science:

Biophysical techniques
Analytical chemistry
Molecular interaction analysis

Background:

Traditional label-free optical detection methods primarily measure molecular mass, limiting sensitivity for small molecules.
The charge sensitive optical detection (CSOD) technique offers a sensitive alternative for detecting molecular interactions, irrespective of molecular size.

Purpose of the Study:

To introduce and detail the CSOD technique for label-free, real-time molecular interaction measurement.
To highlight CSOD's capability in studying small molecules and its compatibility with high-throughput screening.

Main Methods:

Utilizes an optical fiber functionalized with probe molecules within a microplate well.
Applies an alternating electrical field to induce fiber oscillation, driven by surface charge.
Monitors changes in oscillation amplitude and phase via optical imaging of the fiber tip.

Main Results:

CSOD measures molecular binding by detecting alterations in the fiber's surface charge.
The technique demonstrates sensitivity to charge changes, independent of the binding molecule's size.
CSOD is compatible with standard microplate platforms for scalable analysis.

Conclusions:

CSOD provides a sensitive, label-free method for real-time molecular interaction studies, particularly for small molecules.
Its compatibility with microplate platforms facilitates high-throughput screening applications in drug discovery.