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Quantitative and Selective DNA Detection with Portable Personal Glucose Meter Using Loop-Based DNA Competitive
Fei Liu1, Bin Wang2, Yanke Shan2
1Joint International Research Laboratory of Animal Health and Food Safety and Single Molecule Nanometry Laboratory (Sinmolab), Nanjing Agricultural University, Nanjing, China. feiliu24@njau.edu.cn.
Methods in Molecular Biology (Clifton, N.J.)
|November 27, 2021
Summary
This study presents a novel DNA biosensor using a competitive hybridization assay and a personal glucose meter (PGM) for rapid, quantitative DNA detection. The portable device shows high sensitivity and anti-interference, ideal for point-of-care testing in resource-limited settings.
Area of Science:
- Biomedical Engineering
- Molecular Diagnostics
- Biosensor Technology
Background:
- Accurate and rapid DNA detection is crucial for diagnostics.
- Existing methods can be complex and require specialized equipment.
- There is a need for portable, user-friendly biosensors for point-of-care applications.
Purpose of the Study:
- To develop a portable, quantitative, and selective DNA detection biosensor.
- To integrate a loop-based DNA competitive hybridization assay with a personal glucose meter (PGM).
- To enable one-step target DNA recognition and signal reporter generation.
Main Methods:
- Utilized a loop-based DNA competitive hybridization assay.
- Employed invertase-DNA conjugates released via competitive binding with target DNA.
- Quantified glucose generated from sucrose hydrolysis using a personal glucose meter (PGM).
- Incorporated magnetic collection of released conjugates.
Main Results:
- The biosensor demonstrated quantitative and selective DNA detection.
- Achieved target concentration-dependent signal generation through enzyme catalysis.
- Exhibited excellent anti-interference capabilities, even in serum samples.
- The system is easy to operate and suitable for resource-limited environments.
Conclusions:
- The developed DNA biosensor offers a promising strategy for rapid and sensitive DNA detection.
- Its portability, ease of use, and high performance make it suitable for point-of-care testing.
- This technology has significant potential for applications in clinical diagnostics and field testing.
