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Quantitative Bioluminescence Tomography for In Vivo Volumetric-Guided Radiotherapy
Zijian Deng1,2, Xiangkun Xu1,2, Hamid Dehghani3
1Department of Radiation Oncology and Molecular Radiation Sciences, Johns Hopkins University, Baltimore, MD, USA.
Methods in Molecular Biology (Clifton, N.J.)
|November 27, 2021
Summary
This study integrates 3D bioluminescence tomography (BLT) with small animal radiation research platforms (SARRP) for precise tumor irradiation. Quantitative BLT (QBLT) enhances soft tissue targeting accuracy in preclinical radiation therapy research.
Area of Science:
- Preclinical Radiation Oncology
- Biomedical Imaging
- Medical Physics
Background:
- Small-animal irradiators are crucial for preclinical radiation therapy (RT) research, mimicking human treatments.
- Cone-beam computed tomography (CBCT) is the primary imaging modality for guidance but struggles with low-contrast soft tissue targets.
- 2D bioluminescence imaging (BLI) offers high contrast but is insufficient for internal tumor guidance due to optical scattering and absorption.
Purpose of the Study:
- To integrate 3D bioluminescence tomography (BLT) with the small animal radiation research platform (SARRP).
- To introduce quantitative BLT (QBLT) for accurate tumor volume quantification.
- To enable QBLT-guided irradiation for improved soft tissue targeting in small animal models.
Main Methods:
- Integration of QBLT system components with the SARRP.
- Development and application of a detailed calibration protocol for QBLT.
- Step-by-step procedures for conducting QBLT-guided irradiation on small animals.
Main Results:
- Successful integration of QBLT for enhanced soft tissue visualization.
- Demonstration of QBLT's capability in quantifying tumor volume for irradiation planning.
- Establishment of a workflow for QBLT-guided irradiation on the SARRP.
Conclusions:
- QBLT provides a robust solution for accurate soft tissue targeting in small animal radiation research.
- This integrated system overcomes the limitations of CBCT for low-contrast targets.
- QBLT-guided irradiation represents a significant advancement in preclinical RT research accuracy.
