Related Experiment Video
Updated: Oct 11, 2025
09:27
Immobilization of Multi-biocatalysts in Alginate Beads for Cofactor Regeneration and Improved Reusability
Published on: April 22, 2016
17.6K
Immobilization of Urease onto Modified Egg Shell Membrane through Cross Linking
Fatemeh Morovvat1, Seyed Ziae Aldin Samsam Shariat1, Maryam Davoudi2
1Department of Clinical Biochemistry, Isfahan Pharmaceutical Sciences Research Center, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran.
Iranian Biomedical Journal
|November 28, 2021
Summary
Immobilizing urease on PEI-modified eggshell membranes using adsorption or glutaraldehyde cross-linking significantly improves enzyme stability and reusability. These methods enhance urease efficiency for industrial and medical applications.
Area of Science:
- Biotechnology
- Enzyme Engineering
- Materials Science
Background:
- Enzyme immobilization enhances stability and reusability, crucial for industrial applications.
- Urease immobilization efficiency is influenced by membrane type and stabilization methods.
Purpose of the Study:
- To immobilize urease onto PEI-modified eggshell membranes.
- To evaluate the impact of adsorption and glutaraldehyde cross-linking methods on urease properties.
- To assess the storage stability and reusability of immobilized urease.
Main Methods:
- Urease immobilization using adsorption and glutaraldehyde cross-linking on PEI-modified eggshell membranes.
- Characterization via Fourier-transform infrared spectroscopy (FTIR) and Atomic Force Microscopy (AFM).
- Enzyme kinetics measured by the Nessler method; stability and reusability assessed over 21 days at 6 °C.
Main Results:
- FTIR confirmed successful immobilization with new peaks observed.
- Immobilization altered membrane surface roughness and shifted optimal pH.
- Immobilized urease exhibited reduced reaction times, higher Km, and lower Vmax compared to free urease.
- Both immobilization methods improved urease stability and reusability.
Conclusions:
- PEI-modified eggshell membranes offer a promising platform for urease immobilization.
- Adsorption and cross-linking are effective methods to enhance urease efficiency.
- Improved urease performance supports its potential in industrial and medicinal fields.
