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Effect of Ionizing Radiation on the Transcript Variants Expression of p21 Gene

Amir Danyaei1, Ali Teimoori2, Hashem Khanbabaei3

  • 1Department of Medical Physics, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.

Asian Pacific Journal of Cancer Prevention : APJCP
|November 28, 2021
PubMed
Abstract

Insights

Investigating Cyclin-Dependent Kinase 1A (CDK1A) splice variants revealed differential responses to irradiation in cancer cells, highlighting their potential for improving radiotherapy efficacy.

Area of Science:

  • Molecular Biology
  • Genetics
  • Cancer Research

Background:

  • Cyclin-Dependent Kinase 1A (CDK1A) plays crucial roles in cell cycle regulation.
  • CDK1A exhibits multiple transcript variants, each potentially having distinct functions in cellular processes, including cancer.
  • Understanding these variants is vital for targeted cancer therapies.

Purpose of the Study:

  • To investigate the expression patterns of different CDK1A splice variants in various cancer cell lines.
  • To determine the impact of external stimuli, specifically 2 Gy irradiation, on the expression of these variants.
  • To assess the potential of targeting specific CDK1A variants for enhanced therapeutic outcomes.

Main Methods:

  • Utilized A549, MDA-MB-231, and Hek-AD cell lines for experimental analysis.
  • Designed specific primers for three CDK1A variants using Snapgene and BLAST software.
  • Quantified variant expression via RT-qPCR and analyzed the effects of 2 Gy irradiation on expression levels.

Main Results:

  • Primers 1 and 3 for CDK1A variants showed expression in all tested cell lines prior to irradiation.
  • Irradiation led to decreased expression of primer 3 in MDA-MB-231 and A549 cells, with no change in Hek-AD cells.
  • Primer 1 expression varied post-irradiation: a 0.03-fold decrease in A549 cells and no significant change in MDA-MB-231 cells.

Conclusions:

  • Each CDK1A splice variant exhibits unique expression profiles and responses to external stimuli like irradiation.
  • Recognizing the distinct roles of these variants is critical for developing more effective cancer treatment strategies.
  • Targeting specific CDK1A variants holds promise for improving the efficacy of radiotherapy.
Keywords:
Irradiationcyclin-dependent kinase inhibitor1p21transcript variants

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