Related Experiment Video
Updated: Oct 11, 2025
09:43
Subtyping of Campylobacter jejuni ssp. doylei Isolates Using Mass Spectrometry-based PhyloProteomics MSPP
Published on: October 30, 2016
8.7K
Markers for discriminating Campylobacter concisus genomospecies using MALDI-TOF analysis
Stephen L W On1, Junwen Zhang1, Angela J Cornelius2
1Wine, Food and Molecular Biosciences, Lincoln University, PO Box 85084, Lincoln, New Zealand.
Current Research in Microbial Sciences
|November 29, 2021
Summary
Accurate identification of Campylobacter concisus genomospecies is crucial for understanding human illness. Matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) shows promise for differentiating these groups, aiding disease research.
Area of Science:
- Microbiology
- Genomics
- Mass Spectrometry
Background:
- Campylobacter concisus comprises distinct genomically varied groups (genomospecies) with differing pathogenic potentials.
- Current identification methods lack the reliability and accessibility needed to distinguish these groups.
- Understanding the role of C. concisus genomospecies in human health is limited.
Purpose of the Study:
- To evaluate the utility of Matrix-Assisted Laser Desorption/Ionization-Time of Flight Mass Spectrometry (MALDI-TOF MS) for differentiating C. concisus genomospecies.
- To identify potential spectral markers for improved discrimination between C. concisus genomospecies using MALDI-TOF MS.
Main Methods:
- Analysis of 19 well-characterized C. concisus strains using a commercial MALDI-TOF MS system.
- Spectra data mining to identify discriminatory markers between genomospecies.
Main Results:
- The commercial MALDI-TOF MS database could only confidently identify one of the 19 C. concisus strains.
- Analysis of spectral data revealed potential markers for distinguishing between C. concisus genomospecies.
Conclusions:
- Standard MALDI-TOF MS identification is insufficient for differentiating C. concisus genomospecies.
- Careful application and data mining of MALDI-TOF MS analysis show potential for discriminating C. concisus genomospecies.
- This approach could clarify the significance of diverse C. concisus genomospecies in human diseases.
Related Concept Videos
01:19MALDI-TOF Mass Spectrometry
5.8K
Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.
Matrix-assisted laser desorption ionization (MALDI) is a commonly...
Matrix-assisted laser desorption ionization (MALDI) is a commonly...
5.8K
01:29Modern Molecular Taxonomy
250
Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...
250