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Updated: Oct 11, 2025
Thermodynamics of Membrane Protein Folding Measured by Fluorescence Spectroscopy
Published on: April 28, 2011
Measurement of Submillisecond Protein Folding Using Trp Fluorescence and Photochemical Oxidation
David Witalka1, Lisa J Lapidus2
1Department of Physics and Astronomy, Michigan State University, East Lansing, MI, USA.
Abstract:
Observation of protein folding on submillisecond time scales requires specialized ultra-rapid mixers coupled to optical or chemical probes. Here we describe the protocol for employing a microfabricated mixer with a mixing time of 8 μs coupled to a UV confocal microscope. This instrument can detect Trp fluorescence and also excite hydroxyl radicals that label the folding protein which can be detected by mass spectrometry.
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