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Related Experiment Video

Updated: Oct 11, 2025

A Semi-Automated and Reproducible Biological-Based Method to Quantify Calcium Deposition In Vitro

A Semi-Automated and Reproducible Biological-Based Method to Quantify Calcium Deposition In Vitro

Published on: June 2, 2022

CircSamd4: A novel biomarker for predicting vascular calcification.

Yuting Zhou¹, Yehong Liu², Shiyi Xuan¹

¹Wuxi Clinical College of Anhui Medical University, Wuxi, China.

Journal of Clinical Laboratory Analysis

|November 30, 2021

Summary

Vascular calcification (VC) is linked to heart disease. Researchers found that circSamd4a levels decrease in patients with VC, suggesting it could be a useful diagnostic biomarker.

Keywords:

biomarkers circSamd4a circular RNA coronary artery calcification endothelial cells

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Calcification of Vascular Smooth Muscle Cells and Imaging of Aortic Calcification and Inflammation

Calcification of Vascular Smooth Muscle Cells and Imaging of Aortic Calcification and Inflammation

Published on: May 31, 2016

Area of Science:

Cardiovascular Research
Molecular Biology
Biomarker Discovery

Background:

Vascular calcification (VC) is a significant risk factor for cardiovascular diseases (CVDs), a leading global cause of mortality.
Understanding the molecular mechanisms and identifying diagnostic markers for VC is crucial for early intervention.

Purpose of the Study:

To investigate the expression patterns of circular RNAs (circRNAs) in patients with VC.
To evaluate the potential of circRNAs as diagnostic biomarkers for VC.

Main Methods:

Quantitative reverse transcription polymerase chain reaction (qRT-PCR) was used to measure circSamd4a expression in human aortic endothelial cells, mouse aorta, and plasma from VC patients.
Logistic regression analysis and receiver operating characteristic (ROC) curves were employed to assess diagnostic value.

Main Results:

circSamd4a expression was significantly downregulated in calcified human aortic endothelial cells and mouse aorta compared to controls.
Plasma levels of circSamd4a were also significantly lower in patients with VC.
ROC curve analysis indicated that circSamd4a has a substantial diagnostic value for VC (AUC = 0.81).

Conclusions:

circSamd4a exhibits a consistent downregulation across different biological specimens in the context of VC.
circSamd4a demonstrates significant potential as a reliable biomarker for the diagnosis of vascular calcification.