Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

The interaction of daunomycin with polydeoxynucleotides.

D R Phillips, A DiMarco, F Zunino

    European Journal of Biochemistry
    |April 17, 1978
    PubMed
    Summary

    Daunomycin binds tightly to synthetic DNA polymers, showing a preference for alternating sequences. This binding impacts DNA polymerase activity but doesn't correlate with helix stabilization.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    How Well Do We Know the Neutron-Matter Equation of State at the Densities Inside Neutron Stars? A Bayesian Approach with Correlated Uncertainties.

    Physical review letters·2020
    Same author

    Does fluoroscopy prevent inadvertent parathyroidectomy in thyroid surgery?

    Annals of the Royal College of Surgeons of England·2019
    Same author

    Pyrexia in an older man, months after emergency.

    BMJ (Clinical research ed.)·2018
    Same author

    Power counting of contact-range currents in effective field theory.

    Physical review letters·2015
    Same author

    Measurement of Compton scattering from the deuteron and an improved extraction of the neutron electromagnetic polarizabilities.

    Physical review letters·2015
    Same author

    Radioarsenic from a portable (72)Se/(72)As generator: a current perspective.

    Current radiopharmaceuticals·2012

    Area of Science:

    • Biochemistry
    • Molecular Biology
    • Pharmacology

    Background:

    • Daunomycin is an anthracycline antibiotic with known DNA-intercalating properties.
    • Understanding its precise DNA binding interactions is crucial for its therapeutic applications and mechanism of action.

    Purpose of the Study:

    • To investigate the binding affinity and specificity of daunomycin to various synthetic DNA polymers.
    • To correlate DNA binding with functional consequences, such as inhibition of DNA polymerization and thermal stability.

    Main Methods:

    • Quantitative binding measurements using spectrophotometric analysis.
    • Assessment of daunomycin's effect on DNA polymerase I (Escherichia coli) and DNA polymerase alpha (rat liver) activity.
    • Thermal denaturation studies to evaluate DNA double helix stability.

    Main Results:

    • Daunomycin exhibits tight binding to all tested synthetic polydeoxynucleotides.
    • The drug shows equal affinity for dG.dC and dA.dT base-paired sequences.
    • A significant preference for alternating poly(dA-dT).poly(dA-dT) over poly(dA).poly(dT) was observed, indicating sequence-specific binding.
    • Daunomycin binding inactivated the template properties of DNA polymers for DNA polymerases.
    • No correlation was found between binding affinity and heat-induced DNA stabilization.

    Conclusions:

    • Daunomycin interacts strongly with synthetic DNA, with a preference for alternating base sequences.
    • The drug's binding interferes with DNA replication processes mediated by DNA polymerases.
    • DNA binding affinity does not directly predict the stabilization of the DNA double helix against thermal denaturation.

    Related Experiment Videos