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Self-calibrated BFP-type apertometer for both dry and immersion lenses via grating projection reference
Optics Letters
|December 1, 2021
Summary
This study introduces a new method for measuring microscope objective numerical aperture (NA) using multi-references on the back focal plane. This technique enhances measurement range and accuracy, enabling NA measurements above unity.
Area of Science:
- Optical Metrology
- Microscopy Instrumentation
- Nanotechnology
Background:
- Accurate measurement of objective numerical aperture (NA) is crucial for high-resolution microscopy.
- Existing back focal plane (BFP)-based apertometers have limitations in measurement range and accuracy, especially for high-NA objectives.
Purpose of the Study:
- To propose and experimentally validate a novel BFP-type apertometer scheme.
- To achieve a wider measurement range and higher accuracy for objective NA determination.
- To enable measurement of objectives with NA exceeding unity using a virtual Fourier plane.
Main Methods:
- Generating "multi-references" on the BFP using grating projection.
- Implementing a self-calibration mechanism based on these multi-references.
- Utilizing a "virtual" Fourier plane for measurements, particularly for high-NA objectives.
Main Results:
- Demonstrated a wider measurement range for both dry and immersion objectives.
- Achieved higher accuracy through the self-calibration of multi-references.
- Successfully measured objectives with numerical aperture (NA) across unity, a first for BFP-type apertometers.
Conclusions:
- The proposed multi-reference BFP-type apertometer offers superior performance in terms of range and accuracy.
- This novel approach enables the measurement of high-NA objectives previously challenging to characterize.
- The experimental verification confirms the feasibility and effectiveness of the self-calibrated multi-reference apertometer system.

