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Related Experiment Videos

Somatic evolution of variable region structures during an immune response.

L Wysocki, T Manser, M L Gefter

    Proceedings of the National Academy of Sciences of the United States of America
    |March 1, 1986
    PubMed
    Summary
    This summary is machine-generated.

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    Mice immunized with p-azophenylarsonate developed B cells producing high-affinity antibodies. Subsequent exposure amplified antibody diversity through mutation and selection, enhancing antigen binding.

    Area of Science:

    • Immunology
    • Molecular Biology
    • Genetics

    Background:

    • B cells synthesize antibodies with variable (V) regions encoded by gene segments.
    • Antibody V regions are crucial for antigen recognition and binding affinity.

    Purpose of the Study:

    • To investigate the role of somatic mutation and clonal selection in antibody affinity maturation.
    • To understand how antigen exposure shapes the immune repertoire.

    Main Methods:

    • Immunization of mice with p-azophenylarsonate-conjugated protein.
    • Analysis of B cell antibody V regions early and after secondary immunization.
    • Assessment of antibody affinity for p-azophenylarsonate.

    Main Results:

    • A specific V region, initially expressed by a minority of B cells, showed high affinity for the antigen.

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  • Secondary immunization led to the dominance of B cells with mutated V regions, exhibiting even higher affinity.
  • Antigen drives V region diversity amplification through sequential selection and mutation.

    • Conclusions:

    • Antigen directs immune repertoire expression and V region diversity amplification.
    • A sequential process of B cell selection, V gene mutation, and reselection enhances antibody affinity.
    • This mechanism optimizes the immune response for higher affinity antigen binding.