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Adoptive Transfer Immunization of Mice.

Edward A Greenfield

Cold Spring Harbor Protocols

|December 2, 2021

Summary

This study details a method to significantly increase antibody-producing cells. By using gamma irradiation and transferring spleen cells, researchers achieved an 80-fold enrichment for monoclonal antibody generation.

Area of Science:

Immunology
Cell Biology
Biotechnology

Background:

Generating sufficient antibody-forming cells is crucial for producing monoclonal antibodies.
Existing methods may lack the efficiency needed for large-scale antibody production.

Purpose of the Study:

To develop and validate a procedure for enriching antibody-forming cells.
To enhance the yield of antibody-producing cells for monoclonal antibody generation.

Main Methods:

Utilizing gamma irradiation to eliminate the recipient animal's immune system.
Transferring spleen cells, reverted to memory cells, from syngeneic donors to the irradiated recipient.
Allowing the transferred immune cells to proliferate and dominate.

Main Results:

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Achieved an 80-fold enrichment of antibody-producing cells compared to the original immunized animal.
Demonstrated the successful expansion of functional antibody-forming cells.

Conclusions:

This procedure offers a highly effective method for enriching antibody-forming cells.
The technique provides a significant advantage for the generation of monoclonal antibodies.