Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

CD4<sup>+</sup> T cells impair tumor growth through IL-3 and TNF-dependent vascular damage.

Science (New York, N.Y.)·2026

Same author

Macrophage Proteomic Profiling Reveals Divergent TLR4-Dependent and -Independent Responses to Kdo<sub>2</sub>-Lipid A and Lipid IVa.

Life (Basel, Switzerland)·2026

Same author

High-Plex Tissue Imaging with Conventional Immunofluorescence Platforms and Open-Source Software via Iterative Bleaching Extends Multiplexity (IBEX).

Methods in molecular biology (Clifton, N.J.)·2026

Same author

Agonistic anti-CD40 antibody treatment converts resident regulatory T cells into activated type 1 effectors within the tumor microenvironment.

Immunity·2026

Same author

Immunological knowledge.

Nature immunology·2026

Same author

Tumour-brain crosstalk restrains cancer immunity via a sensory-sympathetic axis.

Nature·2026

Same journal

Editorial: Technologies for RNA Detection.

Bio-protocol·2026

Same journal

One-Step Affinity Purification of MarathonRT Reverse Transcriptase for RNA Sequencing Applications.

Bio-protocol·2026

Same journal

Enhanced RNA-Seq Expression Profiling and Functional Enrichment in Non-model Organisms Using Custom Annotations.

Bio-protocol·2026

Same journal

Using Combined Fluorescent In Situ Hybridization With Immunohistochemistry to Co-localize mRNA in Diverse Neuronal Cell Types.

Bio-protocol·2026

Same journal

Stepwise Protocol for Alternative Splicing Analysis in Single-Cell SMART-Seq2 RNA-Seq Data.

Bio-protocol·2026

Same journal

Enriching Bacteria-Specific RNA From Host Samples Before NGS With Transcript-Capture.

Bio-protocol·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Oct 11, 2025

Author Spotlight: Exploring Macrophage Immunometabolism Through Lentiviral Vector-Mediated Gene Manipulation

Author Spotlight: Exploring Macrophage Immunometabolism Through Lentiviral Vector-Mediated Gene Manipulation

Published on: February 16, 2024

Lentivirus-mediated Conditional Gene Expression.

Leah Rommereim¹, Ajay Suresh Akhade¹, Ronald N Germain²

¹Institute for Systems Biology, Seattle, USA.

|December 3, 2021

Summary

This summary is machine-generated.

We developed a lentiviral system for controlled gene expression in THP-1 cells. This method enables stable, inducible expression of Nod-Like receptors (NLRs) for versatile research applications.

Keywords:

Lentivirus NLR NOD1 Stable expression TRE Tetracycline

More Related Videos

Lentivirus-mediated Genetic Manipulation and Visualization of Olfactory Sensory Neurons in vivo

Lentivirus-mediated Genetic Manipulation and Visualization of Olfactory Sensory Neurons in vivo

Published on: May 22, 2011

Stereotaxic Injection of a Viral Vector for Conditional Gene Manipulation in the Mouse Spinal Cord

Stereotaxic Injection of a Viral Vector for Conditional Gene Manipulation in the Mouse Spinal Cord

Published on: March 18, 2013

Related Experiment Videos

Last Updated: Oct 11, 2025

Author Spotlight: Exploring Macrophage Immunometabolism Through Lentiviral Vector-Mediated Gene Manipulation

Author Spotlight: Exploring Macrophage Immunometabolism Through Lentiviral Vector-Mediated Gene Manipulation

Published on: February 16, 2024

Lentivirus-mediated Genetic Manipulation and Visualization of Olfactory Sensory Neurons in vivo

Lentivirus-mediated Genetic Manipulation and Visualization of Olfactory Sensory Neurons in vivo

Published on: May 22, 2011

Stereotaxic Injection of a Viral Vector for Conditional Gene Manipulation in the Mouse Spinal Cord

Stereotaxic Injection of a Viral Vector for Conditional Gene Manipulation in the Mouse Spinal Cord

Published on: March 18, 2013

Area of Science:

Molecular Biology
Immunology
Cell Biology

Background:

Controlling gene expression is crucial for understanding gene function.
Nod-Like receptors (NLRs) play significant roles in cellular processes.
Existing methods for gene expression control can be inefficient or lack flexibility.

Purpose of the Study:

To establish a robust and conditional gene expression system for Nod-Like receptors (NLRs) in THP-1 cells.
To utilize a third-generation lentiviral platform for enhanced safety and gene expression.
To provide a flexible method for stable gene induction in various cell types.

Main Methods:

Engineered a lentiviral expression plasmid with a tetracycline-inducible promoter (TRE) and a selection marker.
Generated lentivirus by co-transfecting HEK293T cells with packaging and envelope plasmids.
Infected THP-1 cells at a low multiplicity of infection (MOI) for single viral integration.
Selected and validated viral integration using qPCR.
Induced gene expression with doxycycline and confirmed via qPCR, immunoblot, and flow cytometry.

Main Results:

Achieved stable and conditional expression of the gene of interest in THP-1 cells.
Demonstrated successful viral integration and robust gene induction.
Validated expression levels through multiple techniques, confirming system efficacy.

Conclusions:

The developed lentiviral expression system provides a stable and controllable method for gene expression.
This platform facilitates the study of genes like Nod-Like receptors (NLRs) in cellular systems.
The system's flexibility supports diverse applications in cell biology and immunology research.