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Updated: May 2, 2026
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High-plex Imaging using Spectral Confocal Microscopy to Minimize Non-specific Tissue Fluorescence
Published on: October 28, 2025
963
High-Plex Tissue Imaging with Conventional Immunofluorescence Platforms and Open-Source Software via Iterative
Ian T Lamborn1, Hiroshi Ichise1,2, Emily Speranza3
1Lymphocyte Biology Section, Laboratory of Immune System Biology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA.
Methods in Molecular Biology (Clifton, N.J.)
|April 30, 2026
Abstract
None:
Iterative bleaching extends multiplexity (IBEX) is an easy-to-use, highly multiplex immunofluorescent tissue imaging method that employs widely available microscopy platforms, commercial reagents, and open-source software. In this article, we describe how to implement this method in a laboratory that has minimal experience with immunohistochemistry.
