Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

FISH - Fluorescent In-situ Hybridization02:07

FISH - Fluorescent In-situ Hybridization

22.1K
Fluorescence in situ hybridization, or FISH, was developed in the early 1980s and has quickly become one of the most widely used techniques in cytogenetics. Labeled probes are used to bind complementary DNA or RNA sequences on a chromosome or in a region within a cell. Earlier, the probes could only be obtained by cloning or reverse transcription of a DNA template. Currently, the probe oligonucleotides can be synthesized synthetically. Additionally, with the advancement of optical techniques,...
22.1K
In-situ Hybridization02:31

In-situ Hybridization

9.7K
In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled probe. The technique was first used in 1969 for the investigation of nucleic acids. It is currently an essential tool in scientific research and clinical settings, especially for diagnostic purposes.
Types of probes and labels
A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...
9.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Fatal Case of Splash Pad-Associated Naegleria fowleri Meningoencephalitis - Pulaski County, Arkansas, September 2023.

MMWR. Morbidity and mortality weekly report·2025
Same author

Unexpected and prominent parvovirus infection in a patient with plasma cell myeloma.

Blood·2020
Same author

Plasma cell myeloma in a patient with Gaucher disease.

Blood·2019
Same author

Late breast cancer recurrence with bone marrow metastases and acute pulmonary hypertension.

Proceedings (Baylor University. Medical Center)·2018
Same author

The utility of B-cell receptor gene rearrangement studies in diagnosing diffuse large B-cell lymphoma with plasmacytic differentiation.

Annals of clinical and laboratory science·2015
Same author

First reported case of Ehrlichia ewingii involving human bone marrow.

Journal of clinical microbiology·2014

Related Experiment Video

Updated: Oct 11, 2025

Zebrafish Whole Mount High-Resolution Double Fluorescent In Situ Hybridization
12:31

Zebrafish Whole Mount High-Resolution Double Fluorescent In Situ Hybridization

Published on: March 25, 2009

23.5K

Fluorescent In Situ Hybridization (FISH).

Jeanette M Ramos1

  • 1Department of Pathology and Laboratory Services, University of Arkansas for Medical Sciences, Little Rock, AR, USA. JMRamos@uams.edu.

Methods in Molecular Biology (Clifton, N.J.)
|December 3, 2021
PubMed
Summary
This summary is machine-generated.

Fluorescent in situ hybridization visualizes nucleic acid sequences in cells and tissues. This technique is crucial for specific diagnoses and offers detailed cellular insights.

Keywords:
Adjunct studyChromosomeDeoxyribonucleic acidFluorescentInterphaseRibonucleic acid

More Related Videos

Detection of Viral RNA by Fluorescence in situ Hybridization FISH
10:16

Detection of Viral RNA by Fluorescence in situ Hybridization FISH

Published on: May 5, 2012

38.9K
Fluorescence in situ Hybridizations FISH for the Localization of Viruses and Endosymbiotic Bacteria in Plant and Insect Tissues
10:38

Fluorescence in situ Hybridizations FISH for the Localization of Viruses and Endosymbiotic Bacteria in Plant and Insect Tissues

Published on: February 24, 2014

23.8K

Related Experiment Videos

Last Updated: Oct 11, 2025

Zebrafish Whole Mount High-Resolution Double Fluorescent In Situ Hybridization
12:31

Zebrafish Whole Mount High-Resolution Double Fluorescent In Situ Hybridization

Published on: March 25, 2009

23.5K
Detection of Viral RNA by Fluorescence in situ Hybridization FISH
10:16

Detection of Viral RNA by Fluorescence in situ Hybridization FISH

Published on: May 5, 2012

38.9K
Fluorescence in situ Hybridizations FISH for the Localization of Viruses and Endosymbiotic Bacteria in Plant and Insect Tissues
10:38

Fluorescence in situ Hybridizations FISH for the Localization of Viruses and Endosymbiotic Bacteria in Plant and Insect Tissues

Published on: February 24, 2014

23.8K

Area of Science:

  • Molecular Biology
  • Genetics
  • Cell Biology

Background:

  • In situ hybridization (ISH) is a powerful technique for detecting specific nucleic acid sequences within their cellular or tissue context.
  • Both enzymatic and fluorescent ISH methods serve as valuable diagnostic adjuncts in various medical fields.
  • Accurate diagnosis often relies on the precise localization provided by ISH techniques.

Purpose of the Study:

  • This chapter focuses specifically on the application and methodology of fluorescent in situ hybridization (FISH).
  • To provide a comprehensive overview of FISH techniques for visualizing nucleic acid targets.
  • To highlight the diagnostic utility of FISH in cellular and tissue analysis.

Main Methods:

  • Utilizes nucleic acid probes designed to hybridize with target sequences.
  • Employs fluorescently labeled probes for direct visualization under microscopy.
  • Covers sample preparation and hybridization protocols for FISH.

Main Results:

  • Enables precise localization of DNA or RNA sequences within cells and tissues.
  • Provides high sensitivity and specificity for target detection.
  • Facilitates the identification of genetic abnormalities and gene expression patterns.

Conclusions:

  • Fluorescent in situ hybridization is an indispensable tool in molecular diagnostics.
  • FISH offers unparalleled spatial resolution for nucleic acid analysis.
  • The technique significantly aids in achieving accurate and specific diagnoses.