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Immunohistochemistry, Quality Control, and Principles of Validation in the Central Nervous System

Verónica Velasco-Vales1, Danny Soria-Céspedes2, Teresa Cristina Cuesta-Mejías2,3

  • 1Departamento de Patología, Hospital Ángeles Lomas, Estado de México, Mexico. verovelascov@gmail.com.

Methods in Molecular Biology (Clifton, N.J.)
|December 3, 2021
PubMed

Keywords:
Central nervous systemGliomasImmunohistochemistryMedulloblastomasQuality controlValidation

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Immunocytochemistry (ICC) and immunohistochemistry (IHC) are techniques that use antibodies to check for specific proteins or antigens in a sample. The technique was first published by Albert Coons in 1941 to detect the presence of pneumococcal antigen in tissue sections from mice infected with Pneumococcus. Immunocytochemistry helps localization of proteins or antigens in individual cells like blood cells, stem cells, etc., while immunohistochemistry does the same for tissue samples.
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