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A multi-functional microfluidic device compatible with widefield and light sheet microscopy.

Regan P Moore1, Ellen C O'Shaughnessy2, Yu Shi1

  • 1Joint Biomedical Engineering Department, University of North Carolina at Chapel Hill, North Carolina State University, Chapel Hill, NC, 27599, USA. legantw@email.unc.edu.

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Summary
This summary is machine-generated.

Researchers developed a new microfluidic device for advanced microscopy. This fluorinated ethylene propylene (FEP) device enables high-resolution imaging, reagent exchange, and sterile cell culture for light sheet microscopy applications.

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Area of Science:

  • Biophysics
  • Microscopy
  • Bioengineering

Background:

  • High-resolution microscopy techniques like light sheet microscopy require specialized sample holders.
  • Existing microfluidic devices may have limitations in optical compatibility, reagent handling, and maintaining sterile conditions.

Purpose of the Study:

  • To develop and characterize a novel microfluidic device for seamless integration with light sheet and super-resolution microscopy.
  • To demonstrate the device's utility for long-term cell imaging, dynamic reagent application, and sterile cell culture.

Main Methods:

  • Fabrication of a 150 μm thick microfluidic chamber with a transparent fluorinated ethylene propylene (FEP) cover.
  • Optical characterization of the FEP cover's refractive index (1.34) for compatibility with water (1.33) and top-down imaging.
  • Demonstration of long-term cell culture, reagent perfusion, and sterile handling.

Main Results:

  • The FEP microfluidic device exhibits excellent optical properties for light sheet microscopy.
  • The device successfully supported long-term imaging of cell growth and differentiation.
  • The system allowed for rapid reagent exchange while maintaining sterile conditions and isolating the sample from imaging optics.

Conclusions:

  • The FEP-based microfluidic device is a versatile tool for advanced microscopy, particularly light sheet microscopy.
  • Its compatibility with super-resolution techniques like lattice light sheet structured illumination microscopy (LLS-SIM) and DNA PAINT is demonstrated.
  • The device offers significant advantages for imaging weakly adherent cells, reagent manipulation, and maintaining sample integrity.