Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Labeling DNA Probes03:31

Labeling DNA Probes

8.6K
DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
8.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Phenocopies of 22q11.2DS: revealing genetic diversity in clinically suspected 22q11.2 deletion syndrome.

Molecular and cellular pediatrics·2026
Same author

Optimized Xanthenium Photocages with Fused Ring Systems for Photoactivated Chemotherapy and G Protein-Coupled Receptor Photopharmacology.

Journal of the American Chemical Society·2026
Same author

Fluorescence Quenching Properties and Bioimaging Applications of Readily Accessible Blue to Far-Red Fluorogenic Triazinium Salts.

Journal of the American Chemical Society·2025
Same author

Double Disguise: Camouflaging Photocages for Bioorthogonally Controlled Conditional Activation.

Journal of the American Chemical Society·2025
Same author

Two Decades of Living with Echinococcus multilocularis: A Clinical Epidemiology Study on Human Alveolar Echinococcosis in Hungary.

Infectious diseases and therapy·2025
Same author

New Click on the Block: Photocaged Hydroxylamine, an Addition to the Light-Activatable Bioorthogonal Toolbox.

Chemistry (Weinheim an der Bergstrasse, Germany)·2025

Related Experiment Video

Updated: Oct 11, 2025

Photoactivated Localization Microscopy with Bimolecular Fluorescence Complementation BiFC-PALM
12:42

Photoactivated Localization Microscopy with Bimolecular Fluorescence Complementation BiFC-PALM

Published on: December 22, 2015

10.1K

Bioorthogonal Ligation-Activated Fluorogenic FRET Dyads.

Evelin Albitz1,2, Dóra Kern1,2, Attila Kormos1

  • 1Chemical Biology Research Group, Institute of Organic Chemistry, Research Centre for Natural Sciences, Eötvös Loránd Research Network, Magyar tudósok krt. 2, 1117, Budapest, Hungary.

Angewandte Chemie (International Ed. in English)
|December 3, 2021
PubMed
Summary

This study introduces a novel energy transfer system for bioorthogonal fluorogenic labeling. The method enhances cyanine dye performance for clearer, multicolor imaging in live cells, even at sub-diffraction resolution.

Keywords:
FRET dyadbioorthogonalfluorogenicmulticolorsingle excitation

More Related Videos

Studying DNA Looping by Single-Molecule FRET
11:27

Studying DNA Looping by Single-Molecule FRET

Published on: June 28, 2014

15.6K
Author Spotlight: Characterizing Novel Enzymes from Extremophiles and Common Pathogens to Understand DNA Repair and Replication
05:33

Author Spotlight: Characterizing Novel Enzymes from Extremophiles and Common Pathogens to Understand DNA Repair and Replication

Published on: July 5, 2024

884

Related Experiment Videos

Last Updated: Oct 11, 2025

Photoactivated Localization Microscopy with Bimolecular Fluorescence Complementation BiFC-PALM
12:42

Photoactivated Localization Microscopy with Bimolecular Fluorescence Complementation BiFC-PALM

Published on: December 22, 2015

10.1K
Studying DNA Looping by Single-Molecule FRET
11:27

Studying DNA Looping by Single-Molecule FRET

Published on: June 28, 2014

15.6K
Author Spotlight: Characterizing Novel Enzymes from Extremophiles and Common Pathogens to Understand DNA Repair and Replication
05:33

Author Spotlight: Characterizing Novel Enzymes from Extremophiles and Common Pathogens to Understand DNA Repair and Replication

Published on: July 5, 2024

884

Area of Science:

  • Chemical Biology
  • Biophotonics
  • Molecular Imaging

Background:

  • Bioorthogonal chemistry enables specific labeling of biomolecules in complex biological systems.
  • Fluorogenic probes offer signal-on detection, reducing background noise.
  • Limitations exist in achieving bright, photostable, and spectrally distinct signals for multicolor imaging.

Purpose of the Study:

  • To develop an energy transfer-based relay system for amplifying bioorthogonal fluorogenicity.
  • To enhance the performance of yellow/red emitting cyanine dyes for live-cell imaging.
  • To enable multicolor imaging and sub-diffraction resolution using a single excitation source.

Main Methods:

  • Design and synthesis of energy transfer dyads coupling blue-excitable coumarins to cyanine dyes.
  • Utilizing Förster Resonance Energy Transfer (FRET) for signal amplification.
  • Application in no-wash bioorthogonal fluorogenic labeling of intracellular structures.
  • Super-resolution imaging using STED (Stimulated Emission Depletion) microscopy.

Main Results:

  • Achieved improved fluorogenicity and photostability of cyanine dyes via the relay mechanism.
  • Demonstrated large apparent Stokes-shifts, leading to reduced background fluorescence.
  • Enabled simultaneous three-color intracellular imaging using a single excitation source.
  • Successfully performed sub-diffraction imaging of cellular structures.

Conclusions:

  • The developed energy transfer relay system significantly enhances bioorthogonal fluorogenic labeling.
  • This approach provides a versatile platform for multicolor and super-resolution live-cell imaging.
  • The method offers improved signal-to-noise ratio and photostability for advanced cellular analysis.