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REV-ERBs negatively regulate mineralization of the cementoblasts
Liangliang Fu1, Min Wang1, Guixin Zhu1
1The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory of Oral Biomedicine Ministry of Education, School & Hospital of Stomatology, Wuhan University, Wuhan, China; Department of Oral Implantology, School and Hospital of Stomatology, Wuhan University, Wuhan, China.
Biochemical and Biophysical Research Communications
|December 3, 2021
Summary
This study reveals that circadian clock proteins, REV-ERBs, are involved in cementogenesis. REV-ERBs negatively regulate cementoblast mineralization by inhibiting Osterix (OSX) expression, offering a potential target for regeneration.
Area of Science:
- Cell Biology
- Chronobiology
- Dental Research
Background:
- The role of the circadian clock in cementogenesis, the process of tooth root cementum formation, is not well understood.
- REV-ERBs are key proteins within the circadian clock machinery.
Purpose of the Study:
- To investigate the function of REV-ERBs in cementoblast proliferation, migration, and mineralization.
- To elucidate the molecular mechanisms by which REV-ERBs influence cementogenesis.
Main Methods:
- Examined REV-ERBα expression in cementoblasts in vivo and in vitro.
- Utilized CCK-8, scratch wound healing, alkaline phosphatase (ALP), and alizarin red S (ARS) assays.
- Assessed mineralization markers including Osterix (OSX), ALP, bone sialoprotein (BSP), and osteocalcin (OCN).
Main Results:
- REV-ERBα showed strong expression in cellular cementum and its mRNA oscillated in cementoblasts.
- REV-ERB activation by SR9009 inhibited proliferation but enhanced migration of cementoblasts.
- REV-ERBs negatively regulated mineralization and attenuated the expression of OSX and its downstream targets.
Conclusions:
- REV-ERBs play a significant role in cementogenesis by negatively regulating cementoblast mineralization through the inhibition of OSX expression.
- This finding presents REV-ERBs as a potential therapeutic target for periodontal and cementum regeneration.
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