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Updated: Oct 11, 2025
Bioluminescent Bacterial Imaging In Vivo
Published on: November 4, 2012
Responsive nanoplatform for persistent luminescence "turn-on" imaging and "on-demand" synergistic therapy of
Bei-Bei Wang1, Li-Xia Yan1, Li-Jian Chen1
1State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China; International Joint Laboratory on Food Safety, Jiangnan University, Wuxi 214122, China; Institute of Analytical Food Safety, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China.
Abstract:
Multifunctional nanotheranostic platforms are emerging for the treatment of bacterial infections. Uncontrollable drug release and poor response in target location leads to inefficient therapy and failure to offer timely antibacterial monitoring. Here, we report a multifunctional nanoplatform that can be triggered by the bacterial microenvironment for effective bacterial killing and high-sensitive persistent luminescence (PL) "turn-on" imaging. Hyaluronic acid (HA) is grafted on the surface of mesoporous silica-coated persistent luminescence nanoparticles (PLNPs@MSN) loaded with cinnamaldehyde (CA). Further in situ growth of MnO2 shells gives PLNPs@MSN@CA-HA-MnO2 (PMC-HA-MnO2). MnO2 shell of PMC-HA-MnO2 can be reduced to Mn2+ by the H2O2 in the bacterial microenvironment to trigger persistent luminescence (PL) "turn-on" imaging along with chemodynamic therapy (CDT). Meanwhile, HA can response to bacterially secreted hyaluronidase to make the packaged CA release controllable and "on-demand". Consequently, PMC-HA-MnO2 enables effective response to bacterial-infected region, ensuring high-sensitive "turn-on" imaging, synergistic CDT, accurate targeting and "on-demand" CA release to give great antibacterial effect. This nanoplatform has great potential for the diagnosis and treatment of multidrug-resistant bacterial infection with high specificity and efficiency.
