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Preparation of Samples for Electron Microscopy

Preparation of Samples for Electron Microscopy

To be visualized by an electron microscope, either transmission or scanning, biological samples need to be fixed (stabilized) so the electron beam does not destroy them and dried thoroughly (desiccated/dehydrated) so the vacuum does not affect them. Fixation needs to be done as quickly as possible because the sample properties will start changing as soon as it is removed from its natural environment. For example, in a tissue sample, the oxygen levels begin decreasing, causing an altered...

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Updated: Oct 11, 2025

Sample Preparation using a Lipid Monolayer Method for Electron Crystallographic Studies

Sample Preparation using a Lipid Monolayer Method for Electron Crystallographic Studies

Published on: November 20, 2021

Sample Preparation using a Lipid Monolayer Method for Electron Crystallographic Studies.

Chloe D Truong¹, Dewight R Williams², Mary Zhu³

¹School of Molecular Sciences, Arizona State University; Center for Applied Structural Discovery, Biodesign Institute, Arizona State University.

Journal of Visualized Experiments : Jove

|December 6, 2021

Summary

This summary is machine-generated.

This study presents a lipid monolayer method for high-resolution macromolecular structure determination using electron crystallography. This technique supports the growth of two-dimensional (2D) crystals essential for advanced imaging and analysis.

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Last Updated: Oct 11, 2025

Sample Preparation using a Lipid Monolayer Method for Electron Crystallographic Studies

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Preparation and Delivery of Protein Microcrystals in Lipidic Cubic Phase for Serial Femtosecond Crystallography

Preparation and Delivery of Protein Microcrystals in Lipidic Cubic Phase for Serial Femtosecond Crystallography

Published on: September 20, 2016

Area of Science:

Structural Biology
Biophysics
Biochemistry

Background:

Electron crystallography enables high-resolution determination of macromolecular structures.
Two-dimensional (2D) crystals of proteins are crucial for high-resolution reconstruction.
Lipid monolayers have been utilized to support 2D crystallization of various proteins.

Purpose of the Study:

To describe a lipid monolayer methodology for cryogenic electron microscopic (cryo-EM) imaging.
To facilitate the 2D crystallization of proteins for structural studies.

Main Methods:

Formation of a lipid monolayer at the air-water interface.
Protein binding to the lipid monolayer to promote 2D array formation.
Transfer of the monolayer with the 2D array onto EM grids for imaging.

Main Results:

The lipid monolayer method supports the growth of ordered 2D crystals.
This technique is applicable to soluble, peripheral, and integral membrane proteins.
The method facilitates sample preparation for cryo-EM imaging.

Conclusions:

Lipid monolayers provide a robust support for 2D crystallization in electron crystallography.
This methodology enhances the quality of 2D crystals for high-resolution structure determination.
The described technique is valuable for advancing cryo-EM studies of macromolecules.