Related Experiment Video
Updated: Feb 28, 2026
11:34
A Competent Hepatocyte Model Examining Hepatitis B Virus Entry through Sodium Taurocholate Cotransporting Polypeptide as a Therapeutic Target
Published on: May 10, 2022
2.7K
Protocol for purifying recombinant HBV capsids from HepG2 cells and comparative phosphorylation analysis by
Katie Culhane1, Shiv Kumar1, Jianming Hu1
1Penn State College of Medicine Department of Cell and Biological Systems, Hershey, PA 17033, USA.
STAR Protocols
|February 26, 2026
Summary
This study details a protocol for purifying hepatitis B virus (HBV) capsids and analyzing their phosphorylation. This method aids in understanding HBV capsid phosphorylation states for viral lifecycle research.
Area of Science:
- Virology
- Molecular Biology
- Biochemistry
Background:
- The hepatitis B virus (HBV) capsid protein (HBc or Cp) is a key component of the viral particle.
- HBc/Cp contains multiple phosphorylation sites that are crucial for regulating different stages of the HBV lifecycle.
Purpose of the Study:
- To present a detailed protocol for the purification of HBV capsids.
- To enable the analysis of the phosphorylation states of purified HBV capsids.
Main Methods:
- The protocol involves cell transfection to produce HBV capsids.
- Purification is achieved using gradient ultracentrifugation.
- Methods include capsid detection, concentration, and phosphorylation-level profiling.
Main Results:
- The described protocol provides a practical approach for assessing the global phosphorylation state of purified HBV capsids.
- The method is adaptable for comparative analyses between different capsid preparations.
Conclusions:
- This protocol offers a valuable tool for researchers studying HBV capsid phosphorylation.
- Understanding capsid phosphorylation is essential for elucidating the HBV viral lifecycle.
