Reprogramming of one human induced pluripotent stem cell line from healthy donor
Shuxing Cao1, Jun Ma2, Jinyu Zhang3
1The Second Hospital of Hebei Medical University, Hebei Province 050017, China.
Abstract:
In this study, skin biopsy was, and the fibroblasts were isolated from the dermal explant cultures. Human induced pluripotent stem cell (iPSC) line was generated from the skin fibroblasts collected from a healthy 50-year old male donor with informed consent. The reprogramming of fibroblasts was performed with four Yamanaka factors containing Oct3/4, Sox2, Klf4 and c-Myc. The generated iPSCs were confirmed integration-free, expressed pluripotency markers, displayed the normal karyotype, and demonstrated trilineage differentiation potential. This iPSC model can be used to model physiological processes and screen drug validation in vitro.
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