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Published on: October 9, 2011
A novel enterovirus in lambs with poliomyelitis and brain stem encephalitis
Herbert Weissenböck1, Arnt Ebinger2, Anna Maria Gager1
1Institute of Pathology, Department of Pathobiology, Vienna, Austria.
Abstract:
An Austrian organic dairy sheep farm experienced cases of recumbency and sudden deaths in 3- to 4-week-old lambs. Two animals were subjected to thorough clinical and pathological investigations. Pathohistological analysis identified severe nonsuppurative myelitis and mild nonsuppurative encephalitis. A reverse-transcription quantitative PCR (RT-qPCR) assay for the recently discovered ovine picornavirus causing comparable lesions scored negative. By next-generation sequencing-based metagenomics, a nearly complete genome of a novel enterovirus could be detected and assembled. In situ hybridization using a specifically designed probe revealed robust signals in affected motoneurons of the spinal cord suggesting a causative role of the novel virus.
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