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Related Concept Videos

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Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
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Spatial-proteomics reveals phospho-signaling dynamics at subcellular resolution.

Ana Martinez-Val1, Dorte B Bekker-Jensen1,2, Sophia Steigerwald1,3

  • 1Novo Nordisk Foundation Center for Protein Research, Proteomics Program, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark.

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|December 8, 2021
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This study introduces a new high-throughput proteomics workflow to map protein and phospho-protein changes across cellular compartments. It reveals dynamic protein relocation during stress signaling, offering insights into cellular responses.

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Area of Science:

  • Cellular Biology
  • Proteomics
  • Signaling Pathways

Background:

  • Dynamic changes in protein location are crucial for cellular responses to stimuli.
  • Current proteomics methods for mapping protein dynamics are often laborious and do not fully capture the phospho-proteome.

Purpose of the Study:

  • To develop and validate a high-throughput workflow for profiling global proteome and phospho-proteome dynamics across subcellular fractions.
  • To investigate spatio-temporal signaling dynamics, including EGFR signaling and stress responses.

Main Methods:

  • Sequential cell fractionation coupled with mass spectrometry-based proteomics.
  • Profiling of global proteome and phospho-proteome across six subcellular fractions.
  • Benchmarking using in vitro (HeLa cells) and in vivo (mouse tissues) models for EGFR signaling.

Main Results:

  • The workflow successfully profiled proteome and phospho-proteome dynamics across subcellular fractions.
  • Spatio-temporal EGFR signaling dynamics were mapped in vitro and in vivo.
  • Revealed cellular relocation of ribosomal proteins in response to hypertonicity and muscle contraction, highlighting stress signaling.

Conclusions:

  • The developed high-throughput workflow enables comprehensive analysis of spatio-temporal protein and phospho-protein dynamics.
  • This approach provides novel insights into cellular signaling mechanisms and stress responses.
  • The data is accessible via https://SpatialProteoDynamics.github.io for further exploration.