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Microbial biosensors are analytical devices that utilize living microbes to detect specific substances through measurable signals. These devices consist of two main components: biosensing organisms and signal-transducing elements. Biosensing organisms, such as Escherichia coli or Saccharomyces cerevisiae, are typically housed in multiwell plates connected to transducers, enabling rapid, real-time detection of target analytes.Signal Generation MechanismWhen a target analyte—such as...
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Printed microfluidic sweat sensing platform for cortisol and glucose detection.

Aditi R Naik1, Yiliang Zhou1, Anita A Dey2

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This study presents a low-cost smart bandage for real-time sweat analysis, enabling continuous glucose and cortisol monitoring. The flexible, printed microfluidic device validates reliable measurements across various sweating rates for personal health.

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Area of Science:

  • Biomedical Engineering
  • Materials Science
  • Analytical Chemistry

Background:

  • Wearable sweat biosensors are crucial for non-invasive health monitoring.
  • Scalable manufacturing requires inexpensive, flexible sensor fabrication.
  • Real-time sweat analysis needs validation at diverse physiological conditions.

Purpose of the Study:

  • To develop a low-cost, "smart bandage" microfluidic platform for simultaneous cortisol and glucose detection in sweat.
  • To validate sensor performance under varying sweating rates using a synthetic skin model.
  • To demonstrate a flexible, disposable, and portable sweat sensing system.

Main Methods:

  • Fabrication of a laser-cut, adhesive-based microfluidic device with inkjet-printed graphene and silver electrodes.
  • Development of an antibody-derived electrochemical cortisol sensor and an enzymatic glucose sensor.
  • Integration with a synthetic skin mimicking human sweat pores and rates, utilizing soft lithography.

Main Results:

  • Cortisol sensor achieved a 10 pM limit of detection with an electrowetting valve.
  • Glucose sensor demonstrated a 0.2–1.0 mM range and 10 μM limit of detection.
  • Reproducible sensor responses were observed at perspiration rates of 2.0 μL min⁻¹ and higher.

Conclusions:

  • The printed microfluidic sweat sensors offer a low-cost, real-time, multi-diagnostic solution for personal health monitoring.
  • The smart bandage platform shows potential for continuous, non-invasive biomarker tracking.
  • This technology advances wearable diagnostics for improved healthcare accessibility.