Performance Evaluation for Repair of HSGc-C5 Carcinoma Cell Using Geant4-DNA

Area of Science:

• Radiation Biology
• Medical Physics
• Computational Biology

Background:

• Track-structure Monte Carlo simulations are crucial for assessing initial DNA damage from irradiation.
• Previous work established a Geant4-DNA application using the two-lesion kinetics (TLK) model to link DNA damage to rejoining kinetics in V79 cells.
• DNA repair varies by cell line, necessitating model parameter adjustments for different cell types.

Purpose of the Study:

• To extend the Geant4-DNA application with a TLK model for evaluating DNA damage repair in HSGc-C5 carcinoma cells.
• To validate the model by comparing simulated and experimental data for cell survival and DNA rejoining kinetics.
• To enable accurate prediction of radiation treatment effects for HSGc-C5 cells.

Main Methods:

• Developed an extended Geant4-DNA application incorporating a TLK model tailored for HSGc-C5 cells.
• Performed experimental measurements of cell surviving fractions and DNA rejoining kinetics for HSGc-C5 cells irradiated with 70 MeV protons.
• Optimized TLK model parameters using simulated initial DNA damage to match experimental observations.

Main Results:

• The TLK model parameters were successfully optimized for fast and slow DNA rejoining.
• Optimized DNA rejoining speeds showed good agreement with experimental measurements.
• The enhanced Geant4-DNA simulation accurately predicted cell survival and DNA rejoining kinetics for HSGc-C5 cells.

Conclusions:

• The adapted Geant4-DNA and TLK model accurately predicts DNA damage repair and cell survival in HSGc-C5 cells.
• This approach provides a valuable tool for evaluating proton and carbon radiation treatment effects.
• The study bridges initial DNA damage assessment with cellular response prediction for specific cell lines.