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Related Experiment Video

Updated: Oct 10, 2025

A Cryo-pulverization Protocol for Processing Mouse Paws to Evaluate Molecular Pathways of Tissue Inflammation in a Collagen Induced Arthritis Model
11:03

A Cryo-pulverization Protocol for Processing Mouse Paws to Evaluate Molecular Pathways of Tissue Inflammation in a Collagen Induced Arthritis Model

Published on: October 30, 2019

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Collagen-Induced Arthritis Mouse Model.

Edward F Rosloniec1,2,3, Karen Whittington1, Amanda Proslovsky1

  • 1Veterans Affairs Medical Center, Memphis, Tennessee.

Current Protocols
|December 10, 2021
PubMed
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This guide details inducing collagen-induced arthritis (CIA) in mice, a rheumatoid arthritis model. It covers collagen preparation, mouse selection, immunization, and arthritis assessment for reproducible research.

Area of Science:

  • Immunology
  • Autoimmunity
  • Rheumatology

Background:

  • The collagen-induced arthritis (CIA) mouse model is a key tool for studying rheumatoid arthritis (RA).
  • Autoimmune arthritis is triggered by immunizing susceptible mice with type II collagen (CII).

Purpose of the Study:

  • To provide a comprehensive protocol for the reproducible induction and evaluation of CIA in mice.
  • To guide researchers in acquiring, handling, and preparing CII, selecting mouse strains, and performing immunization techniques.

Main Methods:

  • Detailed steps for CII acquisition, handling, and preparation.
  • Guidance on mouse strain selection and immunization techniques.
  • Methods for evaluating arthritis incidence and severity, including antibody and T cell responses.
Keywords:
arthritisautoimmunitycollagenmodelmouse

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Last Updated: Oct 10, 2025

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Main Results:

  • Arthritis onset typically occurs 21–28 days post-immunization.
  • The protocol aims to achieve a high incidence of CIA in susceptible mouse strains.
  • Methods for critical evaluation of disease pathology are provided.

Conclusions:

  • This protocol enables reproducible induction of CIA for RA research.
  • It facilitates the critical assessment of disease mechanisms and potential therapeutics.
  • The methods support comprehensive evaluation of immune responses to CII.