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Chitooligosaccharides production from shrimp chaff in chitosanase cell surface display system
Yanli Chen1, Zhenmin Ling1, Tursunay Mamtimin1
1Ministry of Education Key Laboratory of Cell Activities and Stress Adaptations, School of Life Science, Lanzhou University, Tianshuinanlu #222, Lanzhou 730000, Gansu, PR China.
Carbohydrate Polymers
|December 11, 2021
Summary
This study presents a new method for producing chitooligosaccharides (COS) from shrimp waste using engineered bacteria. The process achieved high yields and demonstrated excellent enzyme stability for efficient biopolymer conversion.
Area of Science:
- Biotechnology
- Biorefining
- Microbial Engineering
Background:
- Chitin, a natural biopolymer, is a valuable resource for biorefineries.
- Efficient production of derived products like chitooligosaccharides (COS) is crucial.
- Existing methods for chitin processing can be inefficient or unstable.
Purpose of the Study:
- To develop a novel, high-yield method for producing COS from shrimp waste.
- To utilize cell surface-displayed chitosanase (CHI-1) for enhanced enzymatic activity.
- To optimize co-fermentation conditions for chitin deprocessing and chitin yield.
Main Methods:
- Co-fermentation of Bacillus subtilis and Acetobacter sp. with shrimp chaff.
- Engineering E. coli BL21-pET23b(+)-NICHI for cell surface display of chitosanase (CHI-1).
- Optimization of fermentation parameters including yeast extract, KH2PO4, ethanol, and glucose concentrations.
Main Results:
- Achieved 94% deproteinization and 92% demineralization efficiency.
- Obtained an 18% chitin yield from shrimp chaff.
- Engineered E. coli maintained 81% enzyme activity after 40 days at room temperature.
- Achieved a 41% COS yield with high hydrolysis efficiency using whole engineered cells.
Conclusions:
- The novel co-fermentation method with engineered E. coli provides a stable and efficient route for COS production.
- Cell surface-displayed CHI-1 demonstrates robust activity and stability, outperforming crude enzyme preparations.
- This approach offers a promising strategy for valorizing shrimp waste in next-generation biorefineries.
