Simple SPR-based colorimetric sensor to differentiate Mg²⁺ and Ca²⁺ in aqueous solutions

L-tryptophan functionalized AgNPs were successfully fabricated using a one-pot synthesis method and assessed as a colorimetric probe for rapid and accurate determination of Mg²⁺ ions. The developed sensor showed a selective response towards Mg²⁺ with no interference from Ca²⁺ in the wide concentration range of 1-200 µM. The sensor's response was optimized in the pH range of 9-10, which can be attributed to the protonation of amine groups and their interaction with Mg²⁺ ions. The stability and selectivity of the sensor were examined in different salt (NaCl) and other metal ions, respectively. The L-tryptophan-AgNPs sensor detected Mg²⁺ with the limit of detection of 3 µM, which is way lower than the concentration range of magnesium in human serum (0.75-1.05 mM). The recovery values of the developed sensor were in the range of 96-102% for the determination of Mg²⁺ in urine samples. The obtained performances proved the potential application of the developed sensor for clinical diagnostic of Mg²⁺ ions where an accurate and rapid response is needed.