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DYNAMICS OF ULTRASTRUCTURAL REARRANGEMENTS OF SKELETAL MUSCLE FIBROBLASTS AFTER SIMULATED GUNSHOT SHRAPNEL WOUNDS
R Mikhaylusov1, V Negoduyko2, S Pavlov1
11Kharkiv Medical Academy of Postgraduate Education; Ukraine.
Georgian Medical News
|December 13, 2021
Summary
Fibroblast behavior in gunshot wounds changes over time. Initially, they aid healing, but after 60 days, their synthetic activity decreases, potentially hindering muscle repair.
Area of Science:
- Histology
- Regenerative Medicine
- Trauma Surgery
Background:
- Gunshot shrapnel wounds cause significant soft tissue damage.
- Fibroblasts play a crucial role in wound healing and tissue regeneration.
- Understanding fibroblast dynamics post-injury is key to improving treatment outcomes.
Purpose of the Study:
- To investigate the ultrastructural changes in skeletal muscle fibroblasts.
- To analyze fibroblast rearrangements within the wound canal at different time points after gunshot shrapnel wounds.
Main Methods:
- Experimental modeling of gunshot shrapnel wounds in rabbit femoral muscle.
- Utilizing an EM-125 electron microscope for ultrastructural examination of muscle tissue.
- Analyzing fibroblast morphology at 30 and 60 days post-injury.
Main Results:
- At 30 days, fibroblasts exhibited ultrastructure indicative of compensatory-adaptive, synthetic, and reparative processes.
- By 60 days, fibroblasts in the wound canal showed disrupted submicroscopic organization.
- A decrease in fibroblast synthetic activity was observed at the 60-day mark.
Conclusions:
- Fibroblast function shifts from active repair to reduced synthetic activity over time post-gunshot wound.
- These changes in fibroblast ultrastructure may impact long-term muscle tissue regeneration.
- Further research is needed to explore interventions to sustain beneficial fibroblast activity.
