Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Reporter Genes02:11

Reporter Genes

12.1K
Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
12.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Side-by-Side Systematic Characterization of Novel FPs in Budding Yeast.

ACS synthetic biology·2026
Same author

Pea transcriptional and phytohormonal responses to adapted and non-adapted aphid biotypes at early stages of infestation.

Scientific reports·2026
Same author

Combined "omics" and physiological approaches highlight the roles of the GABA shunt and mitochondria-related functions in rice seed longevity.

Plant physiology and biochemistry : PPB·2026
Same author

A green lifetime biosensor for calcium that remains bright over its full dynamic range.

eLife·2025
Same author

Identification and functional analysis of putative effector proteins from <i>Diaphorina citri</i> and '<i>Candidatus</i> Liberibacter asiaticus'.

Frontiers in plant science·2025
Same author

A phloem-based defense mechanism linked to elevated riboflavin levels in wild tomato Solanum chmielewskii impedes whitefly nymphal development.

The Plant journal : for cell and molecular biology·2025
Same journal

Exploring potential strategies to enhance memory and cognition in aging mice.

F1000Research·2026
Same journal

Construction an Implicit Block Multi-Steps Approach for Solving Sixth-Order Fractional Differential Equations.

F1000Research·2026
Same journal

Kansei Engineering in the Evolving Service Sector: A Decade of Insights.

F1000Research·2026
Same journal

A Safety-First Mindset:  Role of Patient Safety Culture in Enhancing Healthcare Workers' Emotional Intelligence.

F1000Research·2026
Same journal

Decoding Decisions: Personality-Interest Motivational Sequences as Predictors of Career Paths.

F1000Research·2026
Same journal

Beyond the Transparent Barrier: A Domain Visualization and Integrative Review of Contemporary Research on Gender-Based Professional Stasis.

F1000Research·2026
See all related articles

Related Experiment Video

Updated: Oct 10, 2025

Analyzing Tumor Gene Expression Factors with the CorExplorer Web Portal
08:00

Analyzing Tumor Gene Expression Factors with the CorExplorer Web Portal

Published on: October 11, 2019

7.7K

PlotXpress, a webtool for normalization and visualization of reporter expression data.

Elias Brandorff1, Marc Galland1, Joachim Goedhart1

  • 1University of Amsterdam, Amsterdam, The Netherlands.

F1000Research
|December 13, 2021
PubMed
Summary
This summary is machine-generated.

plotXpress offers a free, open-source tool for analyzing gene reporter assay data. This platform standardizes data visualization and analysis, making complex results accessible without coding.

Keywords:
Reporter assaysdata visualizationgene expressionluciferasewebtool

More Related Videos

Optimization of a Multiplex RNA-based Expression Assay Using Breast Cancer Archival Material
11:12

Optimization of a Multiplex RNA-based Expression Assay Using Breast Cancer Archival Material

Published on: August 1, 2018

8.1K
ExCYT: A Graphical User Interface for Streamlining Analysis of High-Dimensional Cytometry Data
05:12

ExCYT: A Graphical User Interface for Streamlining Analysis of High-Dimensional Cytometry Data

Published on: January 16, 2019

11.6K

Related Experiment Videos

Last Updated: Oct 10, 2025

Analyzing Tumor Gene Expression Factors with the CorExplorer Web Portal
08:00

Analyzing Tumor Gene Expression Factors with the CorExplorer Web Portal

Published on: October 11, 2019

7.7K
Optimization of a Multiplex RNA-based Expression Assay Using Breast Cancer Archival Material
11:12

Optimization of a Multiplex RNA-based Expression Assay Using Breast Cancer Archival Material

Published on: August 1, 2018

8.1K
ExCYT: A Graphical User Interface for Streamlining Analysis of High-Dimensional Cytometry Data
05:12

ExCYT: A Graphical User Interface for Streamlining Analysis of High-Dimensional Cytometry Data

Published on: January 16, 2019

11.6K

Area of Science:

  • Molecular Cell Biology
  • Biotechnology

Background:

  • Reporter assays are crucial for studying gene expression levels in molecular cell biology.
  • Current commercial reporter assay kits lack standardized data analysis and presentation methods.

Purpose of the Study:

  • To introduce plotXpress, a free, open-source platform for semi-automated analysis and standardized visualization of gene reporter data.
  • To address the lack of standardization in downstream analysis and presentation of reporter assay results.

Main Methods:

  • plotXpress accepts raw luminescence data from reporter gene assays with internal controls.
  • The platform corrects for sample variation using the internal control and calculates average expression per condition.
  • It computes fold changes relative to a user-selected reference condition.

Main Results:

  • plotXpress generates standardized dot plots with statistical summaries for experimental data.
  • The tool allows for adjustable, publication-grade plot creation without requiring programming skills.
  • It facilitates reproducible analysis and appealing visualization of reporter assay outcomes.

Conclusions:

  • plotXpress is a low-threshold, web-based tool that enhances standardization and reproducibility in reporter assay data analysis.
  • The platform provides accessible, high-quality visualizations for gene expression studies.
  • It empowers researchers to present their findings effectively and efficiently.