First report of Raphanus sativus cryptic virus 2 on cabbage seeds (Brassica rapa subsp. pekinensis) imported from Italy
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Combining Analysis of DNA in a Crude Virion Extraction with the Analysis of RNA from Infected Leaves to Discover New Virus Genomes
Published on: July 27, 2018
Minji Jun1, Sanghyun Shin1, Seong-Jin Lee1
1Plant Quarantine Technology Center, Department of Plant Quarantine, Animal and Plant Quarantine Agency, Gimcheon, 39660 Republic of Korea.
Abstract:
Raphanus sativus cryptic virus 2 (RsCV-2), an unclassified species belonging to the family Partitiviridae, was identified in cabbage seeds (Brassica rapa subsp. pekinensis) imported from Italy using high-throughput paired-end RNA sequencing. Three contigs derived from RsCV-2 isolate PQ shared nucleotide sequence homologies (approximately 94, 87, and 86%) with dsRNA1, 2, and 3 of RsCV-2, respectively. Phylogenetic analysis and pairwise comparison showed that RsCV-2 isolates were more closely related to deltapartitiviruses. To the best of our knowledge, this is the first report of RsCV-2 on B. rapa subsp. pekinensis in the world.
Supplementary Information:
The online version contains supplementary material available at 10.1007/s13337-021-00744-w.
