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Related Experiment Video

Updated: Oct 10, 2025

Detection and Monitoring of Tumor Associated Circulating DNA in Patient Biofluids
06:53

Detection and Monitoring of Tumor Associated Circulating DNA in Patient Biofluids

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A multiplexed circulating tumor DNA detection platform engineered from 3D-coded interlocked DNA rings.

Sha Yang1, Xinyu Zhan1, Xiaoqi Tang1

  • 1Department of Clinical Laboratory Medicine, Southwest Hospital, Third Military Medical University (Army Medical University), 30 Gaotanyan, Shapingba District, Chongqing, 400038, China.

Bioactive Materials
|December 13, 2021
PubMed
Summary
This summary is machine-generated.

A novel platform using 3D-coded interlocked DNA rings enables sensitive, multiplexed detection of circulating tumor DNA (ctDNA) without sequencing. This technology advances early cancer detection and personalized medicine.

Keywords:
3D-coded ID ringsCirculating tumor DNAColorectal cancerMultiplexed detection

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Area of Science:

  • Biomarker Discovery
  • Molecular Diagnostics
  • Nanotechnology

Background:

  • Circulating tumor DNA (ctDNA) is a crucial biomarker for early cancer detection and personalized treatment strategies.
  • Current ctDNA detection methods primarily rely on sequencing, which can be costly and time-consuming.
  • There is a need for more sensitive and efficient methods for multiplexed ctDNA identification.

Purpose of the Study:

  • To develop a novel platform for multiplexed ctDNA identification using 3D-coded interlocked DNA rings.
  • To demonstrate the platform's ability to detect ctDNA from various non-invasive clinical specimens.
  • To achieve higher sensitivity compared to existing sequencing-based methods.

Main Methods:

  • A platform termed three-dimensional-coded interlocked DNA rings (3D-coded ID rings) was engineered.
  • The platform integrates ctDNA recognition rings with reporter rings, utilizing target-responsive cleavage by restriction endonucleases.
  • Rolling circle amplification and internal 3D codes facilitate signal amplification and multiplexed readouts.

Main Results:

  • The 3D-coded ID ring platform successfully detected ctDNAs in non-invasive clinical specimens, including plasma, feces, and urine.
  • Sensitivity achieved was significantly higher than that of sequencing-based methods.
  • The platform demonstrated the capability for simultaneous detection of multiple DNA fragments without sequencing.

Conclusions:

  • The 3D-coded ID ring platform offers a highly sensitive and multiplexed approach for ctDNA detection.
  • This technology surpasses current sequencing limitations for ctDNA analysis.
  • The platform holds significant potential for advancing personalized and precision medicine through improved cancer diagnostics.