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A Human Bone Marrow 3D Model to Investigate the Dynamics and Interactions Between Resident Cells in Physiological or Tumoral Contexts
Published on: December 16, 2022
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A minimal standardized human bone marrow microphysiological system to assess resident cell behavior during normal and
Thibault Voeltzel1,2,3,4,5, Gaëlle Fossard1,2,3,4,6, Michaël Degaud1,2,3,4,6
1CNRS UMR5286, Centre de Recherche en Cancérologie de Lyon, 69000 Lyon, France.
Biomaterials Science
|December 14, 2021
Summary
Researchers created a reproducible 3D bone marrow model using a scaffold and human cells. This microphysiological system allows studying cell behavior and disease in a human bone marrow-like environment.
Area of Science:
- Biomedical Engineering
- Cell Biology
- Hematology
Background:
- The bone marrow is a complex microenvironment crucial for cellular regulation.
- Understanding bone marrow dynamics is key for hematological and non-hematological processes.
Purpose of the Study:
- To develop a standardized, reproducible three-dimensional (3D) model of human bone marrow.
- To create a microphysiological system for studying cellular mechanisms and disease pathology.
Main Methods:
- Utilized a biphasic calcium phosphate-based scaffold with human cell lines.
- Established a well-organized, bone marrow-like structure in vitro.
- Enabled characterization via in situ imaging and viable cell retrieval.
Main Results:
- The 3D model demonstrated a reproducible, organized bone marrow-like structure.
- Various cell types and extracellular matrix were observable and characterizable.
- The system accurately mimicked patient bone marrow changes upon introduction of pathological elements.
Conclusions:
- A flexible and standardized 3D microphysiological system for human bone marrow was developed.
- This model facilitates histological analysis and functional assays on retrieved cells.
- The system aids in deciphering cellular control mechanisms and disease processes in bone marrow.
