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Updated: Oct 10, 2025

Conducting Maximal and Submaximal Endurance Exercise Testing to Measure Physiological and Biological Responses to Acute Exercise in Humans
Published on: October 17, 2018
Acute Physiological Responses to Moderate-Intensity Continuous, High-Intensity Interval, and Variable-Intensity
Justin P Guilkey1, Meral Culver1, Matthew Wolfe1
1Coastal Carolina University.
Abstract:
Purpose: This study examined acute physiological responses to moderate-intensity continuous exercise (MICE), variable-intensity intermittent exercise (VIIE), and high-intensity interval exercise (HIIE) protocol, matched for total work output in healthy adults (n = 8, age = 25.1 ± 6.0 yrs). Methods: Three experimental trials were completed in a random order. MICE was continuous exercise at 40% of peak work rate (WRpeak). VIIE consisted of sixteen 10-sec bursts at 120% WRpeak, sixteen 20-sec bursts at 60% WRpeak and recovery at 20% WRpeak interspersed throughout the protocol. HIIE consisted of eight intervals of 2 minutes at 70% WRpeak and 1 minute at 20% WRpeak. Oxygen consumption during work-intervals and time within moderate and vigorous-intensity zones determined the magnitude and duration of physiological stresses. Perceptual responses were measured by affective response and post-exercise enjoyment (PACES). Repeated-measure ANOVAs analyzed differences between trials. Statistical significance was established if p ≤ 0.05. Results: Oxygen consumption during work-intervals in VIIE was greater than MICE, but less than HIIE. VIIE and HIIE had similar duration within vigorous-intensity zone (11.1 ± 3.5 min vs. 13.5 ± 1.3 min); both were greater than MICE (4.9 ± 1.1 min). The time spent in the moderate-intensity zone was different between all trials (VIIE = 11.1 ± 2.0 min; MICE = 17.4 ± 5.2 min; HIIE = 4.9 ± 0.4 min). Affective responses were similar among all trials. Post-exercise enjoyment was greater in VIIE than HIIE; both were not different from MICE. Conclusion: These results suggests the VIIE paradigm could be a viable alternative to HIIE and MICE protocols.
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