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MicroRNA-573 inhibits cell proliferation, migration and invasion and is downregulated by PICSAR in cutaneous squamous

Yanhua Wang1, Shengjian Tang2, Jianping Lv1

  • 1Department of Aesthetic and Plastic Surgery, Weifang People's Hospital, Weifang, Shandong, China.

Bosnian Journal of Basic Medical Sciences
|December 14, 2021
PubMed

Insights

MicroRNA-573 (miR-573) is decreased in cutaneous squamous cell carcinoma (cSCC) and acts as a tumor suppressor. It inhibits cSCC cell proliferation, migration, and invasion, counteracting the effects of long non-coding RNA PICSAR.

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • Cutaneous squamous cell carcinoma (cSCC) incidence is rising globally.
  • MicroRNAs (miRNAs) are critical regulators in cancer development, including cSCC.
  • The specific role of microRNA-573 (miR-573) in cSCC remains underexplored.

Purpose of the Study:

  • To investigate the expression levels of miR-573 in cSCC.
  • To elucidate the relationship between miR-573 and long non-coding RNA PICSAR in cSCC.
  • To analyze the biological functions of miR-573 in cSCC progression.

Main Methods:

  • Quantitative Real-Time PCR (qRT-PCR) for measuring PICSAR and miR-573 expression.
  • Dual-luciferase reporter assay and Pearson's correlation to confirm PICSAR-miR-573 interaction.
  • Cell Counting Kit-8 (CCK-8) and Transwell assays to assess cell proliferation, migration, and invasion.

Main Results:

  • PICSAR expression was elevated, while miR-573 levels were reduced in cSCC tissues and cell lines.
  • PICSAR directly binds to miR-573, leading to its downregulation in cSCC.
  • Overexpression of miR-573 significantly suppressed cSCC cell proliferation, migration, and invasion.
  • miR-573 overexpression reversed the oncogenic effects of PICSAR in cSCC cells.

Conclusions:

  • miR-573 functions as a tumor suppressor in cSCC by inhibiting cell proliferation, migration, and invasion.
  • PICSAR downregulates miR-573, contributing to cSCC tumorigenesis.
  • miR-573 represents a potential therapeutic target for cSCC treatment.

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