Electrochemiluminescence Aptasensor for Charged Targets through the Direct Regulation of Charge Density in
Yanling Huang1, Fang Luo2, Jian Wang1
1Ministry of Education Key Laboratory for Analytical Science of Food Safety and Biology, Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, College of Chemistry, Fuzhou University, Fuzhou, Fujian 350116, China.
Abstract:
The change of surface charge density can cause many changes in physical or chemical properties and has been applied to design many sensitive sensors. Ochratoxin A (OTA) is a negatively charged target in neutral or alkaline solutions. In this work, a microchannel-based electrochemiluminescence (ECL) aptasensor for OTA detection based on this character had been designed. The charged target directly combined with functionalization layers of the microchannels, which caused surface charge density variation and therefore resulted in the change of ECL intensity of the (1,10-phenanthroline)ruthenium(II)/tripropylamine system. The decrease of ECL intensity is linearly dependent on OTA concentration ranging from 0.5 to 4 ng mL-1 with a detection limit down to 0.17 ng mL-1. This strategy has the advantages of simple interface chemistry design and universality, which offers a guiding significance for the charged target assay.
