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Characterization of Functionally Associated miRNAs in Glioblastoma and their Engineering into Artificial Clusters for Gene Therapy
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CircularLRRC7 is a Potential Tumor Suppressor Associated With miR-1281 and PDXP Expression in Glioblastoma

Xue Kong1,2,3,4, Ruiting Xu4, Wei Wang3,4

  • 1School of Medicine, Shandong University, Jinan, China.

Frontiers in Molecular Biosciences
|December 16, 2021
PubMed

Insights

Circular RNAs (circRNAs) function as tumor suppressors in glioblastoma. Lower expression of circLRRC7 correlates with poor patient survival and suggests a novel therapeutic pathway involving miR-1281 and PDXP.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Circular RNAs (circRNAs) are prevalent in neural tissues.
  • Many circRNAs act as tumor suppressors, with reduced expression observed in gliomas.
  • The specific roles and clinical implications of tumor-suppressive circRNAs in glioblastoma (GBM) are not well understood.

Purpose of the Study:

  • To investigate the role of circLRRC7 as a potential tumor suppressor in glioblastoma.
  • To identify the clinical significance of circLRRC7 expression in GBM patients.
  • To elucidate the molecular pathway regulated by circLRRC7 in GBM.

Main Methods:

  • Bioinformatic analysis of circRNA expression in GBM and normal brain tissues.
  • Experimental validation of circLRRC7 expression levels.
  • Survival analysis (progression-free and overall survival) based on circLRRC7 expression.
  • Prediction and validation of miRNA and mRNA targets using bioinformatic databases and correlation analysis.

Main Results:

  • circLRRC7 was significantly downregulated in GBM tissues compared to normal brain tissues (p < 0.0001).
  • Low circLRRC7 expression was associated with poorer progression-free survival (PFS) and overall survival (OS) in GBM patients (p < 0.05).
  • miR-1281 was identified as a potential downstream target of circLRRC7, and PDXP was predicted as a target of miR-1281.

Conclusions:

  • circLRRC7 exhibits tumor-suppressive properties in glioblastoma.
  • The circLRRC7/miR-1281/PDXP axis represents a potential regulatory pathway in GBM.
  • circLRRC7 may serve as a novel therapeutic target for glioblastoma treatment.
Keywords:
GBMPDXPcircRNAhsa_circ-0114014miR-1281miRNAtumor suppressor

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