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Updated: Oct 9, 2025
Tissue Preparation and Immunostaining of Mouse Craniofacial Tissues and Undecalcified Bone
Published on: May 10, 2019
Histological Techniques for Sectioning Bones of the Vertebrate Craniofacial Skeleton
Daniela M Roth1, Lakshmi Puttagunta2, Daniel Graf3,4
1School of Dentistry, University of Alberta, Edmonton, AB, Canada. dmroth@ualberta.ca.
Abstract:
Histochemical analysis is an indispensable technique in the field of biology used routinely to characterize pathologies of interest throughout the system. This chapter provides the craniofacial biologist with an introduction to tissue harvesting, embedding, and sectioning as well as a toolkit of useful stains for stromal/mesenchymal tissues including bone and cartilage. Techniques are tailored to decalcified, paraffin-embedded mouse tissue; however, these methods are applicable under a broad range of conditions.
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