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Updated: Oct 9, 2025

A Strategy for Sensitive, Large Scale Quantitative Metabolomics
Published on: May 27, 2014
Mass spectrometry based approaches and strategies in bioanalysis for qualitative and quantitative analysis of
Frank Klont1, Gérard Hopfgartner1
1Life Sciences Mass Spectrometry, Department of Inorganic and Analytical Chemistry, University of Geneva, Quai Ernest Ansermet 24, 1211 Geneva, Switzerland.
Abstract:
Mass spectrometry plays an essential role in qualitative and quantitative analysis of pharmaceutically relevant molecules. The present review summarizes some the most common applications of LC-MS for the characterization of therapeutic low-molecular-weight compounds, peptides and proteins, and oligonucleotides using low-resolution and high-resolution tandem mass spectrometry. In addition, the benefit of multistage MS, differential ion mobility, and data independent acquisition is emphasized. At last, the potential of coupling MS with novel interfaces for high-throughput analysis is discussed.
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