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Assessment of Oxidative Damage in the Primary Mouse Ocular Surface Cells/Stem Cells in Response to Ultraviolet-C UV-C Damage
Published on: February 15, 2020
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Urban sediment pollutants alternate human cell essential behaviour through promoting oxidative damage
Meng-Wei Lin1, Hsiao-Lan Huang2, Xin-Ru Yu3
1Institute of Bioinformatics and Structural Biology, National Tsing Hua University, Hsinchu, Taiwan.
Ecotoxicology and Environmental Safety
|December 17, 2021
Summary
Downstream Ke-Ya River sediment extract is cytotoxic to human cells, increasing reactive oxygen species (ROS) and impairing cell viability, adhesion, and motility. This study highlights sediment toxicity risks to human health.
Area of Science:
- Environmental toxicology
- Human cell biology
- Biomedical science
Background:
- Industrialization has increased sediment pollution, raising human health concerns.
- Ke-Ya River sediment, downstream from Hsinchu Science Park, is suspected of contributing to health issues.
- Limited data exists on the human health impacts of sediment pollutants.
Purpose of the Study:
- To develop a human cell-based platform for assessing sediment toxicity.
- To evaluate the effects of Ke-Ya River sediment on oxidative damage and cell behavior.
- To investigate the cytotoxic potential of sediment from different river zones on human cells.
Main Methods:
- Utilized 11 different human cell lines to assess sediment extract effects.
- Analyzed sediment from upstream, middle-stream, and downstream Ke-Ya River locations.
- Quantified reactive oxygen species (ROS) levels and evaluated cell viability (MTT assay), adhesion, and motility.
Main Results:
- Downstream Ke-Ya River sediment extract (DSE) exhibited higher cytotoxicity than middle-stream and upstream extracts.
- DSE significantly increased ROS levels in all tested human cell types.
- DSE reduced cell viability in brain, oral, lung, breast, liver, pancreatic, cervical, prostate, and colorectal cells.
- Cell adhesion and wound healing capabilities were significantly impaired by DSE.
Conclusions:
- Ke-Ya River's downstream sediment poses a significant cytotoxic risk to human cells.
- Elevated ROS levels induced by sediment pollution are linked to impaired cellular functions.
- This research provides a foundation for understanding sediment toxicity and its public health implications.
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