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Related Experiment Videos

Factor XIa-alpha 1 antitrypsin complex--elevation in the patients with DIC.

H Nishikado, Y Komiyama, M Masuda

    Thrombosis Research
    |November 15, 1986
    PubMed
    Summary

    A new assay detects the factor XIa-alpha 1 antitrypsin complex (F.XIa-alpha 1AT complex) in plasma. This assay shows significantly higher complex levels in disseminated intravascular coagulation (DIC) patients compared to healthy individuals.

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    Area of Science:

    • Biochemistry
    • Hematology
    • Immunology

    Background:

    • Factor XIa plays a role in coagulation.
    • Alpha 1 antitrypsin is a serine protease inhibitor.
    • The complex of factor XIa and alpha 1 antitrypsin (F.XIa-alpha 1AT complex) is relevant in hemostasis.

    Purpose of the Study:

    • To develop and validate a novel assay for quantifying the F.XIa-alpha 1AT complex in human plasma.
    • To assess the diagnostic utility of the F.XIa-alpha 1AT complex assay in distinguishing between healthy individuals and patients with disseminated intravascular coagulation (DIC).

    Main Methods:

    • Development of an enzyme-linked differential antibody immunosorbent assay (ELISA) using monoclonal antibodies against factor XI.
    • Quantification of the F.XIa-alpha 1AT complex using a standard curve generated with purified complex diluted in factor XI-deficient plasma.

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  • Measurement of complex levels in plasma samples from normal individuals and DIC patients.

    • Main Results:

    • The assay demonstrated high recovery (>90%) for the F.XIa-alpha 1AT complex.
    • Neither factor XI nor alpha 1 antitrypsin alone produced a colorimetric signal, indicating assay specificity.
    • Normal individuals exhibited undetectable levels (<0.18 ng/assay) of the F.XIa-alpha 1AT complex.
    • Patients with DIC showed significantly elevated levels of the F.XIa-alpha 1AT complex, ranging from 0.18 to 4.2 ng/assay.

    Conclusions:

    • The developed assay is specific and sensitive for detecting the F.XIa-alpha 1AT complex in plasma.
    • Elevated levels of the F.XIa-alpha 1AT complex are associated with disseminated intravascular coagulation (DIC).
    • This assay may serve as a valuable diagnostic tool for DIC.