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The Use of a β-lactamase-based Conductimetric Biosensor Assay to Detect Biomolecular Interactions
Published on: February 1, 2018
Laccase bioconjugate and multi-walled carbon nanotubes-based biosensor for bisphenol A analysis
Iria Bravo1, Mariana Prata2, Álvaro Torrinha2
1REQUIMTE-LAQV, Instituto Superior de Engenharia do Instituto Politécnico do Porto, Rua Dr. António Bernardino de Almeida 431, 4249-015 Porto, Portugal; Departamento de Química Analítica y Análisis Instrumental, Universidad Autónoma de Madrid, 28049 Madrid, Spain; Instituto Madrileño de Estudios Avanzados (IMDEA) Nanociencia, Faraday, 9, Campus UAM, Cantoblanco, 28049 Madrid, Spain.
A novel electrochemical biosensor effectively detects Bisphenol A (BPA), an endocrine disruptor, in aquatic environments. This sensitive and stable sensor utilizes laccase enzyme and carbon nanotubes for accurate environmental monitoring.
Area of Science:
- Environmental Science
- Analytical Chemistry
- Biotechnology
Background:
- Bisphenol A (BPA) is a prevalent endocrine-disrupting compound found in aquatic ecosystems, posing environmental and health risks.
- Accurate and sensitive detection methods are crucial for monitoring BPA levels in water bodies.
Purpose of the Study:
- To develop and optimize an electrochemical biosensor for the determination of Bisphenol A (BPA).
- To enhance biosensor performance using a synergistic bioconjugate and nanostructured electrode materials.
Main Methods:
- Development of a bioconjugate comprising laccase enzyme, chitosan, and an ionic liquid.
- Modification of a screen-printed carbon electrode with multi-walled carbon nanotubes (MWCNTs).
- Electrochemical characterization and optimization of the biosensor for BPA detection.
Main Results:
- The optimized biosensor demonstrated high electrocatalytic activity and stability.
- Achieved a wide linearity range up to 12 µM, sensitivity of 6.59 × 10-2 μAµM-1, and a low detection limit of 8.4 nM.
- The biosensor exhibited excellent reproducibility, stability over one month, and accurate results in river water samples.
Conclusions:
- The developed MWCNT-enhanced electrochemical biosensor offers a highly sensitive, stable, and reproducible platform for BPA determination.
- The biosensor shows significant potential for in situ environmental monitoring of BPA in aquatic ecosystems.
