Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Next-generation Sequencing

Next-generation Sequencing

The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

CSDE1 Associates with TOM20 and Mitochondrial Protein-Encoding mRNAs in Sensory Neurons.

Antioxidants (Basel, Switzerland)·2026

Same author

Structurally engineered ultrasoft PEDOT:PSS fiber microelectrodes with enhanced electrochemical performance for neural interfaces.

Science advances·2026

Same author

Multilayered microelectrode array for monitoring electrophysiological signals of 3d neural networks in cerebral organoid.

Microsystems & nanoengineering·2026

Same author

Miniaturized Bidirectional Thermal Stimulation System Integrated With an Electrode Array for Recording Neural Activities.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026

Same author

Stretchable Microelectrode Arrays with Microneedles for Reliable Electrophysiological Recording of Human Heart and Brain Organoids.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026

Same author

Chemiluminescent ELISA based on enzyme-mediated hydrogen peroxide supply.

Enzyme and microbial technology·2026

Same journal

Large-Area Atomically Flat Monocrystalline Gold Flakes: Recent Advances, Applications, and Future Potential.

Small (Weinheim an der Bergstrasse, Germany)·2026

Same journal

Decoupling Processing-Morphology-Stability Relationships Enables 19.65% Organic Solar Cells With Exceptional Photostability.

Small (Weinheim an der Bergstrasse, Germany)·2026

Same journal

Tunable and Selective Doping Modulation in Pd-Filled Carbon Nanotube Transistors.

Small (Weinheim an der Bergstrasse, Germany)·2026

Same journal

Multifunctional Microgels: From Material Design to Skin Wound Healing Applications.

Small (Weinheim an der Bergstrasse, Germany)·2026

Same journal

A Tissue-Homologous Keratin-PBA Hydrogel Integrating Rationally Designed Nanomicelles Enables Microenvironment-Adaptive Repair of Chronic Diabetic Wounds.

Small (Weinheim an der Bergstrasse, Germany)·2026

Same journal

Modulation of 1D Ru-Porphyrin Biomimetic COF to Enhance Synergistic Dual C─H Bond Air Oxidation for Cyclohexenone Synthesis.

Small (Weinheim an der Bergstrasse, Germany)·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Oct 9, 2025

Infinium Assay for Large-scale SNP Genotyping Applications

Infinium Assay for Large-scale SNP Genotyping Applications

Published on: November 19, 2013

Multiplex SNP Genotyping Using SWITCH: Sequence-Specific Nanoparticle with Interpretative Toehold-Mediated Sequence

Woongsun Choi^1,2, Eunhye Park^3,4, Seojin Bae⁵

¹Department of Chemical and Biological Engineering, Korea University, Seoul, 02841, Korea.

Small (Weinheim an Der Bergstrasse, Germany)

|December 19, 2021

Summary

This summary is machine-generated.

This study introduces a novel PCR-free method for multiplex single nucleotide polymorphism (SNP) genotyping. The SWITCH technology enables accurate detection of multiple SNPs simultaneously using nanoparticle probes and hydrogel decoding.

Keywords:

gold nanoparticles hydrogel microparticles magnetic microparticles multiplex single nucleotide polymorphism genotyping sandwich assays toehold-mediated DNA displacement warfarin

More Related Videos

Candidate Gene Testing in Clinical Cohort Studies with Multiplexed Genotyping and Mass Spectrometry

Candidate Gene Testing in Clinical Cohort Studies with Multiplexed Genotyping and Mass Spectrometry

Published on: June 21, 2018

The Visual Colorimetric Detection of Multi-nucleotide Polymorphisms on a Pneumatic Droplet Manipulation Platform

The Visual Colorimetric Detection of Multi-nucleotide Polymorphisms on a Pneumatic Droplet Manipulation Platform

Published on: September 27, 2016

Related Experiment Videos

Last Updated: Oct 9, 2025

Infinium Assay for Large-scale SNP Genotyping Applications

Infinium Assay for Large-scale SNP Genotyping Applications

Published on: November 19, 2013

Candidate Gene Testing in Clinical Cohort Studies with Multiplexed Genotyping and Mass Spectrometry

Candidate Gene Testing in Clinical Cohort Studies with Multiplexed Genotyping and Mass Spectrometry

Published on: June 21, 2018

The Visual Colorimetric Detection of Multi-nucleotide Polymorphisms on a Pneumatic Droplet Manipulation Platform

The Visual Colorimetric Detection of Multi-nucleotide Polymorphisms on a Pneumatic Droplet Manipulation Platform

Published on: September 27, 2016

Area of Science:

Biotechnology
Molecular Diagnostics
Nanotechnology

Background:

Single nucleotide polymorphisms (SNPs) significantly impact individual phenotypes, disease susceptibility, and therapeutic responses.
Accurate SNP genotyping is crucial but challenging due to allele similarity and low sample concentrations.
Existing methods often require polymerase chain reaction (PCR) amplification, limiting throughput and increasing complexity.

Purpose of the Study:

To develop a novel, PCR-free method for multiplex SNP genotyping.
To enhance the specificity and sensitivity of SNP detection in biological samples.
To provide a simplified platform for simultaneous analysis of multiple SNP targets.

Main Methods:

Development of Sequence-specific nanoparticle with interpretative toehold-mediated sequence decoding in hydrogel (SWITCH) technology.
Utilizing gold nanoparticle probes for sequence-specific transduction of SNP targets into distinct DNA invaders.
Employing toehold-mediated DNA replacement within hydrogel microparticles for decoding invaders via SNP-specific fluorescence signals.

Main Results:

Demonstrated PCR-free amplification, generating approximately 1000-fold signal amplification per SNP target.
Achieved successful 4-plex detection of warfarin-associated SNP targets spiked in human serum.
Exhibited excellent specificity in distinguishing between wild and mutant SNP types without cross-reactivity.

Conclusions:

The SWITCH technology offers a robust and sensitive platform for multiplex SNP genotyping.
This represents the first reported PCR-free, multiplex SNP genotyping assay utilizing a single reporting fluorophore.
The developed method holds significant potential for clinical diagnostics and personalized medicine applications.