Related Experiment Video

Updated: Oct 9, 2025

CRISPR Gene Editing Tool for MicroRNA Cluster Network Analysis
10:40

CRISPR Gene Editing Tool for MicroRNA Cluster Network Analysis

Published on: April 25, 2022

2.6K

Dysregulated Expression of Long Non-Coding RNA MINCR and EZH2 in Colorectal Cancer

Sajjad Cheraghi1, Hamid Asadzadeh2, GholamReza Javadi1

  • 1Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran.

Iranian Biomedical Journal
|December 20, 2021
PubMed
Abstract

Insights

Long non-coding RNA MINCR is overexpressed in colorectal cancer (CRC) tissues, suggesting its potential as a diagnostic biomarker for early cancer detection.

Area of Science:

  • Molecular Biology
  • Oncology
  • Genetics

Background:

  • Long non-coding RNAs (lncRNAs) are critical regulators in human carcinogenesis.
  • lncRNAs, such as MINCR, can interfere with messenger RNAs (mRNAs) like EZH2.
  • The precise roles of lncRNAs in colorectal cancer (CRC) remain unclear.

Purpose of the Study:

  • To investigate the expression levels of lncRNA MINCR and EZH2 mRNA in colorectal tumors.
  • To compare the expression of MINCR and EZH2 in tumor tissues versus adjacent normal tissues.
  • To evaluate the correlation between MINCR and EZH2 expression and clinical parameters in CRC patients.

Main Methods:

  • Real-time PCR was used to quantify lncRNA MINCR and EZH2 mRNA expression.
  • Tissue samples were collected from 114 Iranian patients with colorectal tumors and polyps.
  • Statistical analysis was performed to assess expression levels and clinical correlations.

Main Results:

  • Significant overexpression of both lncRNA MINCR and EZH2 mRNA was observed in CRC tissues compared to control tissues (p < 0.0001).
  • The enhanced expression of MINCR and EZH2 was associated with the pathological stage of CRC.
  • A significant alteration in MINCR expression was noted during CRC development.

Conclusions:

  • lncRNA MINCR expression is significantly altered during colorectal cancer development.
  • MINCR may serve as a potential biomarker for the early detection of CRC.
  • Further research into the molecular mechanisms of MINCR in CRC is warranted.
Keywords:
Colorectal cancerEZH2Long non-coding RNA

Related Concept Videos

lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
9.0K
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
22.2K
Master Transcription Regulators02:23

Master Transcription Regulators

Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
7.2K
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.7K
Epigenetic Regulation01:46

Epigenetic Regulation

Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
31.5K
Non-LTR Retrotransposons03:18

Non-LTR Retrotransposons

As the name suggests, non-LTR retrotransposons lack the long terminal repeats characteristic of the LTR retrotransposons. Additionally, both LTR and non-LTR retrotransposons use distinct mechanisms of mobilization. Non-LTR retrotransposons are further divided into two classes - Long interspersed nuclear elements (LINEs) and short interspersed nuclear elements (SINEs), both of which occur abundantly in most mammals, including humans. Some of the active non-LTR retrotransposons in humans are L1...
12.0K