Differential expression profiling of microRNAs and their target genes during wheat-Bipolaris sorokiniana pathosystem

Wheat spot blotch, caused by Bipolaris sorokiniana, is a serious constraint to wheat production, reducing grain yield and consequently having severe economic impact. Several plant miRNAs have recently been discovered as regulators of gene expression involved in cellular and metabolic functions. So far reports on the roles of miRNAs in B. sorokiniana infection response of wheat are scanty. To further understand the defence mechanism of miRNAs- regulated cellular functions, we examined the expression patterns of 17 miRNAs and their targets involved in the interaction between wheat and B. sorokiniana in two contrasting wheat genotypes, Chiriya-1 and WH-147. All of the miRNAs and target genes were shown to be expressed differentially in both genotypes after B. sorokiniana infection. Seven and nine miRNAs were observed as up-regulated in the resistant genotype Chiriya-1 and the susceptible genotype WH147, respectively. Among the up-regulated miRNAs, ptc-miR901 (~ 10.21 times) accumulated the most in Chiriya-1 followed by ptc-miR1450 (~ 7.6 times) in WH-147. Furthermore, only two miRNAs, tae-miR156 and ptc-miR482c showed a complete inverse relation with their target genes, SPL and NBS-LRR, respectively. This research sheds light on the temporal differential regulation of miRNAs and their targets, which may play a role in wheat adaptation to B. sorokiniana infection.

The online version contains supplementary material available at 10.1007/s12298-021-01092-1.